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In vitro susceptibility of zoospores and hyphae of Pythium insidiosum to antifungals.

The Journal of antimicrobial chemotherapy (2014-02-14)
A O S Fonseca, D I B Pereira, F S Maia Filho, L G Osório, B P Maroneze, J S S Valente, L Pötter, M C A Meireles
ABSTRACT

The purpose of this study was to compare the in vitro susceptibilities of 22 Brazilian isolates of Pythium insidiosum to antifungals using a standardized inoculum of zoospores and a proposed novel inoculum prepared from cultured mycelia (hyphae) of P. insidiosum. A zoospore suspension of P. insidiosum was obtained by the zoosporogenesis technique. The hyphal inoculum was prepared from a suspension of P. insidiosum mycelium. Susceptibility to each drug was evaluated using the CLSI M38-A2 method. Of the 88 MIC comparisons performed, 36 (41%) showed the same MIC value for the two inocula. The agreement (differences not greater than one dilution) between MICs obtained with both types of inocula was 39.8% (35/88). In other MIC comparisons analysed, 17 (19.3%) showed differences of two or three dilutions. We conclude that the use of hyphal inocula of P. insidiosum for in vitro susceptibility tests could be a suitable method for evaluating antimicrobial susceptibility, particularly when it is not possible to obtain a standardized zoospore inoculum.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Itraconazole, ≥98% (TLC)
Itraconazole, European Pharmacopoeia (EP) Reference Standard

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