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New insights into the structure of (1→3,1→6)-β-D-glucan side chains in the Candida glabrata cell wall.

PloS one (2011-11-19)
Douglas W Lowman, Lara J West, Daniel W Bearden, Michael F Wempe, Trevor D Power, Harry E Ensley, Ken Haynes, David L Williams, Michael D Kruppa
ABSTRACT

β-Glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-Glucans provide structural integrity to the fungal cell wall. The nature of the (1-6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to elucidate. Herein, we report the first detailed structural characterization of the (1→6)-β-linked side chains of Candida glabrata using high-field NMR. The (1→6)-β-linked side chains have an average length of 4 to 5 repeat units spaced every 21 repeat units along the (1→3)-linked polymer backbone. Computer modeling suggests that the side chains have a bent curve structure that allows for a flexible interconnection with parallel (1→3)-β-D-glucan polymers, and/or as a point of attachment for proteins. Based on these observations we propose new approaches to how (1→6)-β-linked side chains interconnect with neighboring glucan polymers in a manner that maximizes fungal cell wall strength, while also allowing for flexibility, or plasticity.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Dimethyl sulfoxide-d6, "100%", 99.96 atom % D

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