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  • Mass spectrometry sequencing of long digital polymers facilitated by programmed inter-byte fragmentation.

Mass spectrometry sequencing of long digital polymers facilitated by programmed inter-byte fragmentation.

Nature communications (2017-10-19)
Abdelaziz Al Ouahabi, Jean-Arthur Amalian, Laurence Charles, Jean-François Lutz
ABSTRACT

In the context of data storage miniaturization, it was recently shown that digital information can be stored in the monomer sequences of non-natural macromolecules. However, the sequencing of such digital polymers is currently limited to short chains. Here, we report that intact multi-byte digital polymers can be sequenced in a moderate resolution mass spectrometer and that full sequence coverage can be attained without requiring pre-analysis digestion or the help of sequence databases. In order to do so, the polymers are designed to undergo controlled fragmentations in collision-induced dissociation conditions. Each byte of the sequence is labeled by an identification tag and a weak alkoxyamine group is placed between 2 bytes. As a consequence of this design, the NO-C bonds break first upon collisional activation, thus leading to a pattern of mass tag-shifted intact bytes. Afterwards, each byte is individually sequenced in pseudo-MS

MATERIALS
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Product Description

Sigma-Aldrich
N,N,N′,N′′,N′′-Pentamethyldiethylenetriamine, 99%