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  • A triple stranded G-quadruplex formation in the promoter region of human myosin β(Myh7) gene.

A triple stranded G-quadruplex formation in the promoter region of human myosin β(Myh7) gene.

Journal of biomolecular structure & dynamics (2017-09-21)
Anju Singh, Shrikant Kukreti
ABSTRACT

Regulatory regions in human genome, enriched in guanine-rich DNA sequences have the propensity to fold into G-quadruplex structures. On exploring the genome for search of G-tracts, it was interesting to find that promoter of Human Myosin Gene (MYH7) contains a conserved 23-mer G-rich sequence (HM-23). Mutations in this gene are associated with familial cardiomyopathy. Enrichment of MYH7 gene in G-rich sequences could possibly play a critical role in its regulation. We used polyacrylamide gel electrophoresis (PAGE), UV-Thermal denaturation (UV-Tm) and Circular Dichroism (CD), to demonstrate the formation of a G-quadruplex by 23-mer G-rich sequence HM23 in promoter location of MYH7 gene. We observed that the wild G-rich sequence HM23 containing consecutive G

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Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, ≥98%