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Mechanical characterisation of oocytes - The influence of sample geometry on parameter identification.

Journal of the mechanical behavior of biomedical materials (2017-08-12)
Johannes Dittmann, Andreas Dietzel, Markus Böl
ABSTRACT

The present study focused on the material characterisation of porcine oocytes by experiments combined with an inverse finite-element method (iFEM) approach. In doing so, two different deformation states, compression and indentation, were realised to enable the validation of the numerical model. In addition to classical force-strain relations, geometrical information on the oocyte's surface and volume changes during deformation were collected. These data reveal the typical exponential force-strain behaviour and the highly compressible behaviour of the zona pellucida, imparting overall compressibility to the entire cell. Both force-strain characteristics and geometrical information, along with different combinations of them, were used within the iFEM to identify associated material parameters. As suspected, the identified material parameters show a strong dependence on the information (force-strain relation and/or geometrical information) used in the identification process. Finally, forward finite-element calculations were applied, which verified the quality of the obtained material parameters.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Mineral oil, light oil, (neat)
Sigma-Aldrich
Medium 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture