At the time the User Guide was written, 100 mM was the highest concentration tested. Subsequent testing has been conducted with concentrations as high as 300 mM. Higher concentrations of imidazole are not recommended for concentration. Using imidazole concentrations above 100 mM is not expected to damage the device; however, high salt solutions, including imidazole, may lead to sub-optimal results. Sub-optimal results can include slower flow rates, poorer recovery, and increased detection background or interference in model systems.
UFC9100
Filtro de ultracentrífuga Amicon®, MWCO 100 kDa
sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa
Sinónimos:
Concentrador para centrífuga, MWCO de 100 kDa, volumen de muestra 15 ml
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8 PKG
$137.00
24 PKG
$404.00
96 PKG
$1,570.00
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UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlematerial
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
15 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
12.1 cm (4.807 in.)
diam.
2.97 cm
filtration area
7.6 cm2
volume
15 mL
working volume
≤15 mL
15 mL
pore size
100 kDa MWCO
greener alternative category
shipped in
ambient
1 of 4
Este artículo | UFC210024 | UFC8100 | UFC5100 |
|---|---|---|---|
| material polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing |
| pore size 100 kDa MWCO | pore size 100 kDa MWCO | pore size 100 kDa MWCO | pore size 100 kDa MWCO |
| parameter 15 mL sample volume | parameter 2 mL sample volume | parameter 4 mL sample volume | parameter 0.5 mL sample volume |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
General description
Las unidades de centrífuga Amicon® Ultra de 15 ml con filtros Ultracel® de corte de peso molecular de 100 000 Dalton proporcionan una ultrafiltración rápida y una retención fiable de las macromoléculas. Por ejemplo, la IgG (156 kDa) a una concentración inicial de 1 mg/ml es retenida al >90 %.
El diseño vertical y la superficie de membrana disponible proporcionan un procesamiento rápido de la muestra, una elevada recuperación de la muestra (normalmente superior al 90 % de la disolución inicial diluida) y la capacidad de concentración de 80 veces. La polarización del soluto y el consiguiente ensuciamiento de la membrana se reducen al mínimo gracias al diseño vertical, y un bloqueo físico del filtro evita la centrifugación hasta la sequedad y la posible pérdida de muestra.
El concentrado se recoge del depósito de muestras de la unidad de filtración utilizando un pipeteador, mientras que el ultrafiltrado se recoge en el tubo de centrífuga suministrado. La unidad puede centrifugarse en un rotor oscilante o en un rotor de ángulo fijo. Con su diseño de membrana vertical y bloqueo, los filtros Amicon® Ultra de 15 ml ofrecen la mayor recuperación con tiempos de centrifugado cortos.
El diseño vertical y la superficie de membrana disponible proporcionan un procesamiento rápido de la muestra, una elevada recuperación de la muestra (normalmente superior al 90 % de la disolución inicial diluida) y la capacidad de concentración de 80 veces. La polarización del soluto y el consiguiente ensuciamiento de la membrana se reducen al mínimo gracias al diseño vertical, y un bloqueo físico del filtro evita la centrifugación hasta la sequedad y la posible pérdida de muestra.
El concentrado se recoge del depósito de muestras de la unidad de filtración utilizando un pipeteador, mientras que el ultrafiltrado se recoge en el tubo de centrífuga suministrado. La unidad puede centrifugarse en un rotor oscilante o en un rotor de ángulo fijo. Con su diseño de membrana vertical y bloqueo, los filtros Amicon® Ultra de 15 ml ofrecen la mayor recuperación con tiempos de centrifugado cortos.
Nos hemos comprometido a ofrecerle productos alternativos más ecológicos, que han sido rediseñados para permitir soluciones de investigación más sostenibles. El dispositivo de filtración interior está compuesto por material 60 % biocircular asignado mediante el enfoque de equilibrio de masas ISCC. Pulse aquí para más información.
Application
- Ultrafiltración con membrana Ultracel® de corte de peso molecular de 100 000 dalton.
- Concentración de muestras biológicas que contienen antígenos, anticuerpos, enzimas, ácidos nucleicos o microorganismos.
- Purificación de los componentes macromoleculares encontrados en extractos de cultivo tisular o lisados celulares y eliminación de proteínas antes de la HPLS.
- Concentración de las proteínas diluidas o prepurificadas de los eluyentes de la columna.
- Desalación, cambio de disoluciones tampón y diálisis de proteínas.
Features and Benefits
- La membrana de celulosa regenerada Ultracel® con un NMWCO de 100 kDa proporciona una recuperación de retenido superior al 90 %.
- La membrana vertical reduce la polarización de la concentración para tiempos de centrifugado ultrarrápidos (de hasta 10 – 15 minutos).
- La membrana termosellada minimiza los extraíbles posteriores.
- Integridad probada al 100 % para un rendimiento fiable.
- Cómoda supervisión de la muestra con carcasa traslúcida y gradaciones de volumen.
- El acceso directo a la muestra con pipeteador elimina la etapa de procesamiento para recuperar el concentrado.
- Factores de concentración elevada de 80 – 100 X.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Contenido relacionado
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I see the device is only compatible less than 100 mM imidazole. However the his-tagged protein usually washed higher than 100 mM imidazole. what will happen if it is used to concentrate sample with imidazole higher than 100 mM?
1 answer-
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Can it be fit into 50ml centifuge rotor
1 answer-
Yes, Amicon Ultra-15 units are compatible with adapters for 50 ml tubes.
Helpful?
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Filters with smaller (MWCO) values (3K/10K) tend to produce a higher filtrate volume in a shorter time compared to those with larger MWCOs (e.g., 100K).Why do filters with smaller MWCOs appear to elute faster, even though they have smaller pore sizes?
1 answer-
Each liquid being concentrated in centrifugal devices, each type of substance in the liquid, each amount of particles in a liquid, and each initial volume, type of size, and surface area of a device is unique. Centrifuges are spun at a specific speed based on which type of centrifuge is being used. Therefore, there are many variables involved in the reduction of volume from one initial volume to another, and thus, the end result of the time it takes to reduce volume is different. We as a vendor cannot comment on a customer's specific observation and final result explaining volume reduction and time of a sample.
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The product comes with the tube and filter as one piece. Is this correct? how can i get under 100kDa liqud?
1 answer-
No, the filter component is removable to facilitate sample recovery.
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Temperature limit?
1 answer-
The Amicon Ultra devices are rated for Room Temperature environments and "normal laboratory conditions". We have not studied a set limit but do know that if stored in a warehouse at higher temperatures and humidities, the devices can be affected.
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Is it reusable? How many times I can fill it up again? Amicon Ultra 15. First time I fill it up with 15 ml, and after can I use it again for other 15 ml....and again?
1 answer-
The Amicon Ultra 15 is designed for single use but can be used for repeated spins with the same sample. However, repeated use will cause clogging of the membrane, leading to slower flow rates and longer spin times.
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Hello, Are these tubes valid for AAV concentration? And if so, are these valid for all serotypes? Also, the user guide says that the membrane's material is 'Ultracel® low binding regenerated cellulose'. What's the difference with the PES membrane?
1 answer-
These devices can be used for virus concentration but are not validated for any specific type. Both PES and regenerated cellulose membranes have low binding, but PES has a faster flow rate. For more information, please refer to the technical article on the website: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/viral-concentration-amicon-ultrafiltration
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Hi, I have plan to use the Amicon Ultra-15 100K for Exosome extraction from cell culture supernatant, could you please let me know what would be the best speed (rpm/rcf) and time for doing that? Thanks
1 answer-
Typically, Amicon-15 devices are spun at 4,000 x g (relative centrifugal force) for exosome isolation. However, spin times may need to be optimized, based on the sample type and volume. The image below demonstrated the relationship between volume and spin times. Please see the link below to review the product User Guide. More specific spin time information is available on page 3.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdfHelpful?
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What is the minimum starting volume I can load on Ultra 100K (100,000 MWCO) devices? Can I load as little as 500 µL?
1 answer-
This product is intended for use with 4-15 mL initial sample volumes. The device has not been evaluated for use with volumes smaller than those recommended. While it may be possible to concentrate samples as low as 500 uL, the recovery volume cannot be predicted. Please see the link below to review an alternative Amicon device specifically designed for 500 uL samples.
https://www.sigmaaldrich.com/product/mm/ufc5100Please see the following link to review the full list of 100 kDa MWCO Amicon Ultra products:
https://www.sigmaaldrich.com/substance/amiconultracentrifugalfilter100kdamwco1234598765Helpful?
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Does the Amicon Ultra has residues that may released into the concentrated product', like Beta-Glucan?
1 answer-
These devices contain trace amounts of glycerine. If this material interferes with downstream analysis, rinse the device with buffer or Milli-Q® water prior to use. Should interference continue, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q® water. NOTE: Do not allow the membrane to dry out following rinse steps. If the product will not be used immediately after rinsing, leave the final buffer or water on the membrane until the usage. Please see the link below to review the full product User Guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdfHelpful?
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