Rat collagen solution can be used as a coating for human cells, as indicated by various publications. The following publication in PubMed suggests that rat collagen can serve as a viable alternative to human collagen for various applications in cell culture and tissue engineering. https://pubmed.ncbi.nlm.nih.gov/18203067/
C3867
Rat Collagen Type I
from rat tail, liquid, 4 mg/mL, suitable for cell culture
Collagen, Type I solution from rat tail
Sinónimos:
Colágeno from rat tail, Col1a1, Colágeno de cola de rata
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1 VIAL
$294.00
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Colágeno, tipo I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered
biological source
rat tail
Quality Level
description
Approx. 100mg/vial ((35 - 40 ml) )
sterility
sterile-filtered
product line
BioReagent
assay
>95% (SDS-PAGE)
form
liquid
mol wt
apparent mol wt 115-130 kDa by SDS-PAGE (doublet)
apparent mol wt 215-235 kDa by SDS-PAGE (doublet)
packaging
pkg of 1 vial
concentration
>0.5 mg/mL Biuret
technique(s)
cell culture | mammalian: suitable
surface coverage
6‑10 μg/cm2
impurities
Mycoplasma Test by Barile, none detected
solubility
soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid)
NCBI accession no.
UniProt accession no.
binding specificity
Peptide Source: Collagen
Peptide Source: Fibronectin
shipped in
wet ice
storage temp.
2-8°C
Gene Information
rat ... Col1a1(29393)
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Este artículo | C9791 | C6745 | C8919 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility aseptically processed |
| biological source rat tail | biological source bovine (calf) skin | biological source human cell culture | biological source bovine (calf) skin |
| concentration >0.5 mg/mL Biuret | concentration - | concentration 0.3 mg/mL | concentration (0.1% solution in 0.1 M acetic acid) |
| assay >95% (SDS-PAGE) | assay - | assay - | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
El colágeno es una proteína de matriz extracelular que consta de una estructura helicoidal triple. La estructura del colágeno tipo I, en particular, se caracteriza por la presencia de tres cadenas α idénticas en su forma homotrímera. Entre estas tres cadenas α, una exhibe una ligera variación de la secuencia.[1] El colágeno tipo I se aisló por primera vez del tendón de la cola de rata, tiene dos cadenas α principales (α y α) y una cadena β.[2]
Application
Se ha utilizado una disolución de colágeno tipo I de cola de rata para recubrir placas de cultivo de la línea celular de hepatocitos humanos HepG2[3] y de podocitos.[4] Se ha utilizado como patrón para la generación de la curva de calibración del contenido total de colágeno destinada a su cuantificación en geles de matriz extracelular del hígado.[5]
Se utiliza como material de recubrimiento para soportar el crecimiento y la diferenciación de las células adherentes.
Biochem/physiol Actions
La proteína colágeno es esencial para la la integridad mecánica de los tendones y el hueso. El colágeno del tendón de cola de rata se utiliza en la ingeniería de tejidos en especial en la generación de geles basados en armazones 3-D.[6] Tiene poca antigenicidad y es compatible con los fibroblastos gingivales y los queratinocitos bucales humanos.[2]
Features and Benefits
- Procedente de la cola de la rata.
- Esterilización mediante filtración para reducir al mínimo los riesgos de contaminación.
- Adecuado para una amplia variedad de aplicaciones de cultivo celular.
- Proporciona una cobertura óptima de la superficie (6-10 μg/cm2) para promover la adhesión y la proliferación celulares.
Preparation Note
Disolución estéril preparada a partir de tendones de cola de rata mediante una modificación del método de extracción publicado 1. Se suministra como una disolución acuosa en ácido acético 20 mM con una concentración proteica como la indicada en el frasco. (Aprox.100 mg de proteína por vial). La concentración de proteínas se estimó según el método de Biuret 2.
El análisis de electroforesis en gel de poliacrilamida SDS muestra el típico patrón de bandas para el colágeno tipo I, con un doblete en los pesos moleculares aparentes de 115 y 130 kDa y otro doblete en 215 y 235 kDa. Según este análisis, la pureza de la muestra de colágeno es >95 %.
El análisis de electroforesis en gel de poliacrilamida SDS muestra el típico patrón de bandas para el colágeno tipo I, con un doblete en los pesos moleculares aparentes de 115 y 130 kDa y otro doblete en 215 y 235 kDa. Según este análisis, la pureza de la muestra de colágeno es >95 %.
Se recomienda su uso como sustrato de cultivo celular. Puede no ser adecuado para la formación de gel 3-D.
Other Notes
Cuando C3867 se saca por primera vez del frigorífico aparecerá gelificado. Cuando se le permite llegar por completo a los 37°C, aparecerá en forma líquida.
El colágeno se clasifica en una serie de distintos tipos desde el punto de vista estructural y genético. Nosotros utilizamos la nomenclatura propuesta por Bornstein y Traub. No confundir las designaciones de tipo de Sigma con los tipos de la clasificación reconocida de colágeno.
related product
Referencia del producto
Descripción
Precios
Clase de almacenamiento
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Type I collagen extracted from rat-tail and bovine Achilles tendon for dental application: a comparative study
Techatanawat S, et al.
Asian Biomedicine : Research, Reviews and News, 5(6), 787-798 (2011)
Thermal helix-coil transition in UV irradiated collagen from rat tail tendon
Sionkowska A and Kaminska A
International Journal of Biological Macromolecules, 24(4), 337-340 (1999)
Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications
Rajan N, et al.
Nature Protocols, 1(6), 2753-2753 (2006)
On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids
Mattei G, et al.
Testing, 12(3), e0173206-e0173206 (2017)
Mechanisms of angiotensin II signaling on cytoskeleton of podocytes
Hsu HH, et al.
Journal of Molecular Medicine, 86(12), 1379-1394 (2008)
Artículos
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
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Can rat collagen solution be used as coating for human cells or is it better to use human collagen solutions?
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Should I add collagen I to acetic acid to obtain 0.1% (w/v) collagen solution, or it's already added and I should only dilute it to working concentration which is 0.01?
1 answer-
Here are our preparation instructions:
This product is prepared from rat tail tendons by modification of a published procedure.
It is supplied as an aqueous solution in 20 mM acetic acid (~100 mg of protein per vial). Protein concentration was estimated by the biuret method.
The vial contains approximately 100 mg of protein. Volume per vial: 22–25 ml. The product ships on wet ice and storage at 2–8 °C is recommended. During shipment, or if the storage temperature for this product goes slightly below the recommended range, the product may acquire a gel-like appearance. Under such circumstances, it is possible to warm the product gently, to no more than 45 °C, in order to reconstitute the product fully, before use. Aliquots that have been diluted to a working concentration may be stored at 2–8 °C for up to 2 weeks. Do not freeze. Optimal conditions for attachment must be determined for each cell line and application.1. An appropriate volume of the collagen solution should be diluted to a working concentration of 0.01% using sterile, tissue culture grade water.
2. Coat dishes with 6-10 mg/cm2. Allow the protein to bind for several hours at room temperature or 37 °C, or overnight at 2–8 °C.
3. Remove excess fluid from the coated surface and allow it to dry overnight. If sterility has been compromised, the dried, coated surface can be sterilized easily by overnight exposure to UV light in a sterile tissue culture hood.
4. Rinse with sterile tissue culture grade water or a balanced salt solution before introducing cells and medium.Helpful?
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What will be the concentration of collagen? I am trying to download the CoA, but I am unable to download it.
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This product is prepared at a concentration of approximately 4 mg/mL. A package of 1 Vial will typically have a volume of approximately 35-40 mL. The exact concentration is lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/c3867#product-documentationHelpful?
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IVD conformity: Does this product have IVDD or IVDR conformity?
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This collagen is intended for research use only, and is not approved for IVD purposes.
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In what volume should I dilute this solution in order to make collagen-coated plates for tissue culture?
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The recommended surface coverage for this product is 6?10 μg/cm2. Collagen solutions may be diluted to a working concentration of 0.01%. A general collagen coating protocol is available at https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/collagen-product-protocols
The collagen concentration of this product is reported on a lot specific basis and will be required to prepare the working solution. See the link below to review a sample Certificate of analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C3867-1VL0000255665.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I make a 3-D collagen gel?
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Not all lots of C7661 are suitable for 3D gels. Product No. C4243 has been use-tested in this application. If C4243 is not available, we recommend that customers screen lots of C7661 to try to make 3D gels. We have found that collagen that has been sterilized by gamma-irradiation or by chloroform cannot be used to make 3D collagen gels. The following protocol can be used to make collagen gels: Materials required:Collagen gel solution 1.5 - 3 mg/mL 10X tissue culture medium containing phenol red Sodium bicarbonate or HEPES buffer Procedure: 1.Measure out 800 μL of collagen solution. 2.Add 100 μL of 10X medium (buffered with 1X sodium bicarbonate or HEPES) 3.Adjust pH with 1 N sodium hydroxide, if required (100 μl or less) 4.Add 10X medium to bring volume to 1 ml 5.Mix contents well. Solution should maintain red color to indicate physiological pH6. Dispense into wells to a depth of 1-2 mm (approx. 15 μL/well) 7.Transfer to 37 °C for 20-40 minutes 8.Examine for gel formation The above volumes represent quantities for use in 24 well plates. Volumes can be adjusted to accommodate any culture vessel.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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How are collagen solutions stored?
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Collagen solutions should be stored in the refrigerator at 2-8 °C for up to 1 year, unless it becomes contaminated.
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Why am I seeing some insolubles in my collagen solution?
1 answer-
The solubility specifications for this product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.
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