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MilliporeSigma

SH-SY5Y Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94030304, human nerve, Neuroblast

Sinónimos:

SHSY5Y Cells, SK-SH-SY5Y Cells, SY5Y Cells

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UNSPSC Code:
41106514

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biological source

human nerve

packaging

tube of 5 μg 94030304-DNA-5UG
pkg of vial of cells 94030304-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Neuroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Categorías relacionadas

Application

SH-SY5Y cell line human is used for neurotransmitter studies
SH-SY5Y cell line human has been used to:
  • study the iron-mediated toxicity of amyloid β peptide[1]
  • check the significance of anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA) in vital staining of cells[2]
  • study the effect of haloperidol on sigma-1 receptors in guinea pig brain and human neuroblastoma cells[3]

Biochem/physiol Actions

Human neuroblastoma
SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (Sigma catalogue no. 86012802). SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

Ham′s F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 15% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm2; using 0.25% Trypsin or Trypsin/EDTA, 5% CO2; 37°C. Cells may reattach slowly and may remain in suspension for several days. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.

Other Notes

  • DNA is stored at -70 °C
  • RNA is stored at -70 °C
  • Cell line is stored at -196 °C
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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Questions

1–2 of 2 Questions  

  1. Has this SHSY-5Y cell line been verified as Mycoplasma negative?

    1 answer

    1. Yes, this cell line as supplied is verified as Mycoplasma negative by polymerase chain reaction (PCR), using Mycoplasma-specific PCR primers.

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  2. Hello, I would like to ask about this SH-SY5Y line (94030304). I am using them for testing proliferative capacity (pasage 14-16). If they are not differentiated, are they acting as neuronlike or more cancerous like? Thank you.

    1 answer

    1. Undifferentiated SH-SY5Y cells typically resemble immature catecholaminergic neurons. These cells are characterized by markers indicative of proliferation, such as proliferating cell nuclear antigen (PCNA), as well as by immature neuronal markers such as nestin. Evidence shows that through continuous subculture (or passaging), SH-SY5Y cells start to lose their neuronal characteristics and the potential to generate neurites. The consensus is that cells should be maintained below passage (P) 20. It is therefore important that you manage your stocks of the cell line appropriately. Typically, SH-SY5Y cells are supplied at around P12. It is recommended to subculture the cells at a ratio of 1:10 or higher, seeding at a maximum of 1E4 cells/cm2 and that cryopreserved working stocks of the cells be prepared within two passages of receipt (i.e. around 30 vials at P14). Using this strategy, splitting at a split ratio of 1:10, each vial of working stock would be capable of being expanded 1E6 (up to 1 million times) before the arbitrary P20 limit. Seeding at higher split ratios (up to 1:100) and lower seeding densities (as low as 1,000 cells/cm2) can further maximize the use of these cells.

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