Customer Education and Training

Biology and Analytical Chemistry Webinar Guide

Biology Webinar Guide
Title Topics Covered Length
Multiplex immunoassay detection of Alzheimer’s disease biomarkers in cerebrospinal fluid, plasma, and serum Immunoassay, Biomarkers, Cerebrospinal fluid, Neurodegeneration, Alzheimer’s Disease 40 minutes
SMC™ Technology: Detect Biomarkers at Levels Previously Undetectable Molecules, Immunoassays, Nucleic acids, Protein, Biomarkers 47 minutes
Custom Assay Development and Services utilizing Single Molecule Counting (SMC™) technology SMC, SMCxPro, Erenna, Protein assays, Single Molecule Counting 35 minutes
Tools for improving yields and purity from recombinant bacterial proteins Recombinant, bacterial proteins, detergent based lysis, protein yield, dual purification strategies 37 minutes
Stable Isotope-Labeled Protein Internal Standards Webinar Protein Reference MS LC-MS Standard 60 minutes
Enzyme Explorer Library: Navigation and Application Enzyme explorer, metabolite, activity, library 68 minutes
Single Step Lysis and Purification cell lysis, protein purification, protein extraction 20 minutes
Winning Westerns western blot, protein, troubleshooting, immunoblotting, electrophoresis 60 minutes
Human Protein Atlas Webinar proteome, antibody, biomarker 36 minutes
High Precision PCR utilizing ThermaGenix Reagents PCR, polymerase chain reaction, thermastop, thermago, molecular biology, Genomics 46 minutes
Primer and Probe Design
primer, probe, assay design
7 minutes
An Introduction to qPCR Concepts gene expression, mRNA, assay design 10 minutes
Selecting a qPCR Basic Detection Chemistry assay design, molecular beacons, scorpion probes, locked nucleic acid probes 13 minutes
Choosing a Fluorophore/Quencher Combination assay design, probe design 12 minutes
Chemistries for More Challenging qPCR Assays assay design, optimization, probe design 15 minutes
MIQE Concepts assay standardization, assay reproducibility 3 minutes
Reference Gene Validation normalization, reference gene, NormFinder, GeNorm, GenEX 13 minutes
Data Analysis Guidelines absolute quantification, relative quantification, statistical analysis 11 minutes
MIQE: Assay Design Considerations MIQE, assay design, sample quality, optimization 18 minutes
MIQE: Sample Derived Inhibitors sample quality, inhibitors, assay design, SPUD assay 13 minutes
MIQE: RNA Quality Considerations RNA quality, assay design, integrity assay 16 minutes
MIQE: RNA Quantity and RT Considerations RNA quantification, reverse transcription 17 minutes
PCR Webinar Series: Basic Principles of PCR, qPCR and ddPCR PCR, qPCR, ddPCR, choice of detection method 53 minutes
PCR Webinar Series: qPCR Assay Design evaluation of pre-designed assays, assay design, amplicon and transcript selection, primers, probe design, reverse transcription protocols 56 minutes
PCR Webinar Series: Importance of Sample Quality in qPCR Analysis sample quality 51 minutes
PCR Webinar Series: Improving Assay Quality by Optimization and Validation assay optimization, assay validation 50 minutes
PCR Webinar Series: Data Analysis qPCR data analysis, absolute quantification, relative quantification, expression profiling 61 minutes
PCR Webinar Series: Troubleshooting assay troubleshooting 61 minutes
CRISPR-Based Screening CRISPR revolutionized gene editing, but multi-target screening remains a complex goal. In addition, the fast pace of CRISPR technology development has brought sophisticated options for library screens including new delivery methods for CRISPR gene knockout and the possibility of genome-wide, epigenetic target gene modulation. In this talk, we will explore CRISPR screening technologies for gene knockout, activation, and repression in projects ranging in size from use of small gene panels to whole genome pools and arrayed libraries. 26 minutes
Primary T-Cell Editing using Nickase RNPs In this webinar, we will be discussing some of our most recent testing using our Cas9 proteins, Cas9 RNP nickases in a variety of human cell types, including primary T-cells. Cas nucleases when delivered in protein format, pre-complexed with a single, modified guide RNA (gRNA), rather than encoded on a plasmid, allow researchers to bypass the historically limiting factors of using CRISPR in the therapeutic realm of off-targeting effects, low efficiency, and undesired cellular responses to foreign DNA. Modifying that Cas9 nuclease into a nickase with only a single active cutting domain, and then delivering two with proximal guide RNAs in a pair allows precise double stranded breaks, and provides hyper-specific and efficient genome editing in cell types that are more difficult to edit in. 29 minutes
New CRISPR Strategies in Visualization and Chromatin Accessibility This webinar discusses how formally inaccessible regions of the genome can now be targeted with proxy-CRISPR and CRISPR-chrom technologies as well as how Cas9 proteins tagged with fluorescent markers allow easy visualization of Ribonuclearprotein (RNP) without sacrificing activity
35 minutes
SygRNA® Synthetic CRISPR This webinar discusses the development of the SygRNA® system, protocol optimization, and proposes workflows that enable scientists to quickly incorporate CRISPR technologies into their research 58 minutes
Cas9 Proteins In this webinar, Yanfang Jiang discusses current CRISPR protein products focusing on the specific enzyme characteristics of each protein and their potential applications for the research community. These innovative new proteins are great additions to the researcher’s genome editing workflow. Benefit from Yangfang’s expertise in this field and learn how these new proteins can enhance your CRISPR experimental results 32 minutes
SAM CRISPR Activator Libraries CRISPR-Cas9 nucleases have revolutionized genome editing enabling unprecedented efficiency of targeted mutagenesis. The ability to perform large-scale, whole genome loss-of-function screens has allowed for the rapid identification of gene pathways and targets relevant to drug resistance and disease. The CRISPR SAM activator libraries extend the reach of whole genome screening beyond simple knockout, delivering transcriptional co-regulators to a specific human or mouse target sequence using modified dCas9/gRNA complexes. In this webinar, we will introduce new strategies for forward genetic screening using CRISPR SAM activator libraries.  We will discuss the application of this technology as it pertains to experimental design, delivery mechanisms, data analysis, and target validation 42 minutes
Sanger Whole-Genome Arrayed CRISPR Screening Library CRISPR Cas9 nucleases have revolutionized the field of gene editing and high-throughput lentiviral screens continue to hold ever-increasing promise for both basic research and development of future therapies to benefit human health. Even with such powerful technologies at hand, researchers new to the field may find screening of multiple targets to be challenging and time-consuming. This webinar discusses the Evotec partnership with MilliporeSigma and the screening services for drug discovery 49 minutes
Beyond the Cel-1 Assay: Advancements  in the Gene Editing Workflow Gene editing has become a firmly established technology within the discovery sciences arena.  With the advent of CRISPR/Cas9 systems, the researcher's ability to find an active nuclease for nearly any region of any genome is now a reality. Even with better nucleases available, those who routinely use gene editing tools to manipulate cell lines encounter other significant challenges that pose a barrier to building the "correct" cell line model. As a well-established partner for custom cell line engineering, we at MilliporeSigma have encountered many of these challenges and have developed and/or implemented a number of methods to circumvent them. Several of these methods and the new tools available for gene editing will be discussed in this webinar, along with a summary of how they have impacted our internal cell line engineering program 58 minutes
Genome Editing in Plants with CRISPR/Cas9: Design to Validation plant biotechnology, life sciences, plant research, CRISPR/Cas9 17 minutes
DESIGN. ENGINEER. INNOVATE. Exciting New Applications for CRISPR-Cas9 Proteins CRISPR, Protein, Gene editing, DNA, Biotechnology, Biopharma, plasmid, lentivirus, RNA, Pooled screening 31 minutes
Beyond the Cel-1 Assay: Advancements in the Gene Editing Workflow Cel-1, Cell Line, CRISPR, Nuclease, Gene editing 57 minutes
Methods for Validating a Gene Editing Approach to Treating Brain Disease with Zinc Fingers, TALEs, and CRISPR/Cas9 CRISPR, Cas9, TALEs, ZFN, Genome Editing, Neuroscience, quantitative Western blot 60 minutes *broadcast
November 9, 2016
Register now*
Designing the Next Phase in Genome Editing: Advanced CRISPR Applications CRISPR, Cas9, gRNA, Genome Editing 43 minutes
Pooled CRISPR Screening for Functional Genomics CRISPR, pooled screening, cell culture, FACS, genome editing 50 minutes
CRISPR Paired Nickases for Genome Editing CRISPR/Cas9, Specificity, Paired Nickase 29 minutes
Practical Utilization of CRISPR in the Laboratory Workflow CRISPR, Cas9, Genome Editing, Cell Engineering 33 minutes
Pooled CRISPR Screening for Functional Genomics CRISPR, pooled screening, cell culture, FACS, genome editing 49 minutes
Genome Editing with CRISPR Systems CRISPR/cas9, genome editing 61 minutes
SygRNA®-Synthetic Two Part CRISPR RNA System Cas9, RNA, Genome Editing, sgRNA sequencing 58 minutes
New CRISPR Strategies in Visualization and Chromatin Accessibility CRISPR Visualization with GFP Cas9 Proteins, CRISPR Chromatin Accessibility, and Proxy CRISPR 35 minutes
Cell Culture    
The Value of Cell Line Controls Validation assays, Histology, Quality Control, Histology, Tissue 40 minutes
Getting Your Buffers Right: How to Control the pH of Cell Culture Medium Buffers, Best Practices for Culture Media, Controlling pH, Physiological Buffers 43 minutes
3D Organ-on-a-Chip Applications Using the AIM Biotech Chip 3D cell culture, Organ on chip, Disease model, Researcher, In vitro modeling, Microfluidics, Microfluidic chip, DILI, Toxicity 30 minutes
High-Throughput Microfluidic Platform for Culture of 3D-Kidney Tissue Models Drug toxicity, High-throughput culture, 3D Cell Culture, Cytotoxicity, Efficacy 37 minutes
Current State and Future Prospects for Primary Human Hepatocytes in Basic Research Hepatocytes, Primary cells, Cell-based, Liver 61 minutes
Lighten Up! Long-term imaging with ultra-bright, organic AIE cell trackers AIE, aggregation-induced emission 30 minutes
3D Nephrotoxicity applications & Nortis' microfluidic organ-on-chip technology

3D Nephrotoxicity applications using an in vitro kidney proximal tubule cell based model on a microphysiological system.

Nortis’s microfluidic organ-on-chip technology

120 minutes
Advanced Cell Culture Technology for Generation of In Vivo-like Tissue Models
2D vs 3D cell culture debate, Review of alternative approaches and the development of new technologies, Challenges facing 3D culture methods, in terms of technologies available and methods used, Showcase applications where 3D technology makes a difference, Future perspective for 3D cell culture technology and further development 60 minutes
New 3D-Printable Hydrogel-Based and Particle-Based Biomaterial Ink Platforms Hydrogel bioprinting, 3D-Painting, organ engineering 75 minutes
3D Cell Culture Innovations hanging drop, encapsulation 57 minutes
iPSCs - The Future of Disease Research   40 minutes
Cell Design Studio   75 minutes
Modifying Cell Lines for Target Discovery and Validation cell line engineering 27 minutes
Duolink® PLA Technology: How to detect and quantify protein interactions Protein, Cellular localization, Amplifying, Flow Cytometry 67 minutes
Duolink® PLA Technology: How to Detect and Quantify Protein Interactions Duolink, Protein, Protein-Interaction, PLA 51 minutes

Amplified Detection of Proteins and their Interactions using the Duolink® Proximity Ligation Assay

Proximity ligation assay, Duolink, immunofluorescence, microscopy, immunohistochemistry 60 minutes

Introduction to Duolink and PLA Technology

Duolink, PLA, Protein Interaction

45 minutes

Integrating the Proximity Ligation Assay (PLA) into your Research Program

Antibody, PLA, Validation

48 minutes

Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer

EGFR, Cancer, Diagnostics

52 minutes

Visualizing DNA Methylation (5mC) on a Specific Genomic Locus (SEPTIN9) in Individual Human Cancer Cells usingIn-situ Hybridization and Proximity Ligation Assays

Duolink, Methylation, SEPTIN9

36 minutes

Duolink in Biomedical Science: A Technique for Unraveling the Molecular Mechanisms and Uncovering the Hidden Ultrastructural Features of Gap Junctions

PLA, Gap Junctions, Subcellular Localization

51 minutes

Detection, quantification and visualisation of protein interactions for hit profiling by high content screening

PLA, Duolink, Protein Interaction

59 minutes

Knockdown, Knockout, Validate: Lentivirus delivers payload in vitro and in vivo Lentivirus, Screening, Cell lines, Gene Overexpression, Gene Knockdown/knockout, In vivo 35 minutes
Generation of a landing-pad T cell line useful for T cell receptor customization T cell, Cancer, Immune regulation, CRISPR/Cas9, T cell receptor, AAVS1 Locus, Cell lines 48 minutes

Need to validate your genome editing experiment? LentiORF to the rescue!

LentiORF, Gene Overexpression, Gene Knockdown/Knockout, RNAi, CRISPR, Clones 46 minutes

The Future of RNAi Screening: Complementary Technologies and Advancements using CRISPR and LentiORFs

Screening, LentiORF, Rescue

50 minutes

Decreasing miRNA Sequencing Bias Using a Single Adapter and Circularization Approach

RNA, miRNA, Sequencing, Molecular biology, Biomarkers


55 minutes
Quality Systems and Regulatory Compliance
Title Topics Covered Length
Regulatory Compliance
Unannounced Audits: Are You Ready?
European Commission, Medical Manufacturing, Medical Devices
34 minutes
Drug Discovery Webinar Guide
Title Topics Covered Length
In Vitro Transporter Models for ADME-Tox Research Drug transporter, Biotechnology, drug toxicity, in vitro assay, ATPase 61 minutes
Filtration in Dissolution Testing: Improving Throughput and Reducing Variability Filtration, drug discovery, FRET filters, membrane filters, sample preparation 53 minutes
Late Stage Functionalization of Drug-Like Molecules
drug discovery, C-H functionalization, structure activity relationships
61 minutes
High-capacity drug-delivery systems for cancer therapy based on poly(2-oxazoline)s POx, polymer therapeutics, drug delivery, paclitaxel, hydrophobic, drug loading 71 minutes
Small-Molecule Probes and Inhibitors
Navigating the Promise and Perils of Chemical Probes Inhibitor, Chemical Probes, Protein, Signal Transduction 47 minutes
Sulfonyl Fluorides in Chemical Biology and Drug Discovery Webinar Chemical biology, Sulfonyl fluorides, Chemical probes, Drug discovery, Target identification 60 minutes
Analytical Webinar Guide
Title Topics Covered Length
SPE/SPME/Air Monitoring
Removal of Phospholipids and Proteins from Plasma Using HybridSPE plasma, sensitivity, column life 5 minutes
The Analysis Workflow of the New EPA Method 325 for Fenceline Monitorin Industrial – Petroleum, Oil & Gas Refineries, D & T, Environmental Laboratories - Air 51 minutes
Miniaturization of Sample Handling in Bioanalysis by SPME SPME, high throughput, 96 well 49 minutes
Introduction to SPME (Solid Phase MicroExtraction) SPME, applications 69 minutes
Introduction to Air Monitoring passive, active, methods, equipment 60 minutes
The Extraction of Glycols from Water Using Supelco ENVI-Carb Plus glycol removal, methods 8 minutes
Chapter GC
Unique Selectivity of Ionic Liquid GC Stationary Phases Ionic Liquid Columns, GC Column, Cations, Anions, Ionic Solvents, Stationary phase 54 minutes
Expanding the Capabilities of GC and MS with ionic Liquids complex mixtures, water quantitation, food/beverage analysis 41 minutes
PAH Analysis with GC Columns - Most Popular Columns GC Columns, HPLC, Hydrocarbons, Environments 15 minutes
Evaluations of a New ionic Liquid Staionary Phase with PEG Selectivity GC, thermal stability 48 minutes
Ionic Liquid GC Columns for the Quantification of Trans Fatty Acids in Fats and Oils capillary column, complex lipid separation 31 minutes
More Success in LC-MS – Tips and Tricks for Sample & Mobile Phase Preparation and Column Selection Identification, Sample, Column, Mobile Phase, HPLC Columns 45 minutes
HPLC Column Fundamentals separation, resolution 28 minutes
HPLC Instrument Bandwidth and Optimizing Systems for High-Performance sample dispersion, flow rate, bandwidth 14 minutes
Using LC/MS to Meet the Challenges of a High Through-put Clinical Diagnostic Lab LC/MS, applications, clinical diagnostics 41 minutes
The Use of Alternate Selectivity in Reversed-Phase HPLC RP phases, separation chemistry 43 minutes
Analysis of Contaminants & Competition: Handling R&D Roadblocks method development, case studies 29 minutes
A Practical 2D-LC System Developed for Complex Pharmaceuticals LC x LC, construction, application 31 minutes
Applying Best-in-class HPLC Column Technologies to the Analysis of Proteins and Biotherapeutics protein HPLC, peptide HPLC, biotherapeutics 60 minutes
Applying Best in Class Size Exclusion and Hydrophilic Interaction Chromatography Technologies to the Analysis of Glycoproteins SEC-MS, HILIC-MS, Glycan Analysis 57 minutes
Using Silica HPLC Columns in HILIC Mode: A Practical Guide silica, HILIC, applications, methods 44 minutes
Retention Mechanisms in HILIC: Robust Method Development HILIC, selectivity, method development 55 minutes
HPLC Method Development and Transfer Utilizing Fused-Core Particle Technologies fused-core, column efficiency 31 minutes
Ultra-Fast LC/MS/MS in Bioanalysis Using Fused-Core Columns high throughput, methods, applications 39 minutes
HPLC of Peptides: Speed and Resolution with Fused-Core Columns fused-core, capacity, efficiency 24 minutes
Introducing Ascentis Express 5 um HPLC Columns with Fused-Core Technology Ascentis Express, HPLC, pressure, speed, resolution 52 minutes
Introduction to Ascentis HPLC Columns Ascentis column, small molecule separation 23 minutes
Introducing Ascentis Express F5 with Applications and Theory F5, methods, applications 54 minutes
Introduction to Ascentis Express HPLC Columns fused-core, speed, methods, UHPLC 10 minutes
Introducing Ascentis Express ES-Cyano HPLC Columns ES-Cyano, retention, selectivity 37 minutes
Rapid LC/MS/MS-based Methods for Confirmatory Analysis of Opiates and Benzodiazepines LC/MS, applications, forensics 26 minutes
Ultra-High Performance using Fused-Core HPLC Columns HPLC, LC/MS, sensitivity 28 minutes
Other Analytical Topics
USP : Methods for Moisture Determination and Karl Fischer Titration Karl Fischer Titration, USP, Moisture Determination, Titrimetic, Azeotropic, Gravimetric 31 minutes
Need to judge your product? Use HPTLC to get insights into identity, purity, quantity and sample integrity: one analytical method - easy workflow – comparable result Thin-Layer Chromatography, HPTLC, Pharmacopoeia, cGMP, Botanicals 55 minutes
Making new connections – An overview of new TLC-MS Applications TLC-MS, Sample composition, Coupling techniques 47 minutes
Total Nitrogen Test Kits as an Alternative to TKN, Nitrate, and Nitrite Tests Total nitrogen, Nitrate tests, Nitrite tests, EPA, Spectrophotometric 30 minutes
Introduction to Karl Fischer Titration product development, applications, water content determination 62 minutes
Certified Testing Materials & Proficiency Testing in the 17025 Laboratory reference material, testing, proficiency 46 minutes
QC Reference Materials Pharma Analytical Workflow, QC, Reference Materials, Standards 56 minutes
How to Use Compressed Gas Standards to Calibrate your Thermal Desorption Gas Chromatography System Volatile Organic Compounds, Thermal Desorption, Gas Chromatography 41 minutes
Materials Science Webinar Guide
Title Topics Covered Length
Emerging Quantum Dot Materials: Synthesis and Application Luminescent, Nanoparticle, Emission, Absorption, Photodector, Solar cells 39 minutes
Nano- and Microfabricated Hydrogels for Regenerative Engineering Regenerative Engineering, Polymer Chemistry, Nanotechnology, Functional Scaffolds, Hydrogels, Tissues 66 minutes
Introduction to Gold Nanoparticles - Properties and Applications in Life Science optical and physical properties of gold nanoparticles, surface modification strategies, biomedical applications 44 minutes
Graphene-Based Nanomaterials for Versatile Biosensors electronic and chemical properties, reduced grapheme oxide (rGO), crumpled grapheme (CG), and vertically-oriented grapheme (VG) 61 minutes
Graphene and new monoatomic materials: using 2-dimensional nanosheets in a 3-dimensional world Graphene, supramolecular chemistry, 2D to 3D, 2D meta-materials, conductive, insulating, nanosheets 73 minutes

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