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C8051

Collagenase from Clostridium histolyticum

Sigma Blend Type H, ≥1.0 FALGPA units/mg solid

Synonym(s):

Clostridiopeptidase A

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Size/SKUAvailabilityPrice
100 mg

Estimated to ship onDecember 21, 2026fromMILWAUKEE

$441.00
500 mg

Estimated to ship onSeptember 08, 2026fromMILWAUKEE

$1,250.00
1 g

Estimated to ship onSeptember 08, 2026fromMILWAUKEE

$3,200.00

About This Item

CAS Number:
9001-12-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
MFCD00130830
EC Number:
3.4.24.3 (BRENDA, IUBMB)
Specific activity:
≥1.0 FALGPA units/mg solid

$441.00

Estimated to ship onDecember 21, 2026Details

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Quality Segment

200

form

powder

caseinase activity

≥10 units/mg solid

specific activity

≥1.0 FALGPA units/mg solid

mol wt

68-130 kDa

storage temp.

−20°C

Application

Collagenase from Clostridium histolyticum has been used:
  • to harvest osteosarcoma 143B cells from collagen scaffold
  • in tissue digestion of islets and carotid artery
  • in the digestion of chorionic villi
  • in enzymatic dissociation for ATP-based tumour chemosensitivity assay (ATP-TCA) in solid tumors

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL is used for digestion. The duration of reaction varyies from 15 minutes to several hours to yield a satisfactory efficiency of cell dissociation, without causing too much cell death. Krebs Ringer buffer with calcium and BSA is preffered and Zn2+ is required for activity. This enzyme is tested for the release of hepatocytes at approximately 1 mg/mL conc., in a total volume of 100 mL for each rat liver.
Clostridium histolyticum produces two classes of collagenases. Clostridial collagenases are found to be highly effective than mammalian collagenases. It is capable of cleaving at multiple cleavage sites within the collagen triple helix. It is implicated in the bacterial invasion in gas gangrene.

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

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This Item
C8176C1639C5894
Collagenase from Clostridium histolyticum Sigma Blend Type H, ≥1.0 FALGPA units/mg solid

Sigma-Aldrich

C8051

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum Sigma Blend Type L, ≤1.0 FALGPA units/mg solid

Sigma-Aldrich

C8176

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum 0.2 μm filtered, for general use, Type I-S, 0.2-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

C1639

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum non-sterile; 0.2 μm filtered, Type IA-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

C5894

Collagenase from Clostridium histolyticum

description

Sigma Blend Type H, ≥1.0 FALGPA units/mg solid

description

Sigma Blend Type L, ≤1.0 FALGPA units/mg solid

description

0.2 μm filtered, for general use, Type I-S, 0.2-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

description

non-sterile; 0.2 μm filtered, Type IA-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

specific activity

≥1.0 FALGPA units/mg solid

specific activity

≤1.0 FALGPA units/mg solid

specific activity

≥125 CDU/mg solid, 0.2-1.0 FALGPA units/mg solid

specific activity

≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid

form

powder

form

powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

68-130 kDa

mol wt

68-130 kDa

mol wt

68-130 kDa

mol wt

68-130 kDa

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

caseinase activity

≥10 units/mg solid

caseinase activity

≥40 units/mg solid

caseinase activity

-

caseinase activity

-

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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