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D2001

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Type VIII

Synonym(s):

DNA

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5 MG

PLN 1,960.00

10 MG

PLN 3,490.00

25 MG

PLN 6,920.00

PLN 1,960.00

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About This Item

CAS Number:
93384-16-8
UNSPSC Code:
41106305
eCl@ss:
32160414
EC Number:
297-226-7
NACRES:
NA.51
MDL number:
MFCD00130922

Key Documents

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Product Name

Deoxyribonucleic acid sodium salt from Escherichia coli strain B, Type VIII

type

Type VIII

form

fibers

impurities

<2% protein

Quality Level

200

color

, white to white with yellow cast

solubility

water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow

λmax

256-264 nm (15mM NaCl+1.5mM Trisodium Citrate PH7)

storage temp.

2-8°C

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1 of 4

This Item
D4889D1501D1626
Deoxyribonucleic acid sodium salt from Escherichia coli strain B Type VIII

Sigma-Aldrich

D2001

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Deoxyribonucleic acid sodium salt from Escherichia coli strain B Genomic, unsheared

Sigma-Aldrich

D4889

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Deoxyribonucleic acid sodium salt from calf thymus Type I, fibers

Sigma-Aldrich

D1501

Deoxyribonucleic acid sodium salt from calf thymus

Deoxyribonucleic acid sodium salt from salmon testes

Sigma-Aldrich

D1626

Deoxyribonucleic acid sodium salt from salmon testes

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

form

fibers

form

lyophilized powder

form

fibers

form

solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

<2% protein

impurities

-

impurities

-

impurities

-

type

Type VIII

type

-

type

Type I

type

-

Application

Escherichia coli DNA has many uses in research. Escherichia coli DNA (ecDNA) is used in studies of DNA binding agents that modulate DNA structure and function. E. coli DNA is used in physicochemical studies of DNA behavior in solution, and to evaluate species-specific DNA structures and binding domains. This DNA is particularly useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used to measure the melting temperature (Tm) and in differential scanning calorimetry (DSC) studies.[1] It has also been used as a template sequence in the polymerase chain reaction (PCR).[2]

Preparation Note

Prepared by procedure of Marmur, J., J. Mol. Biol., 3, 208 (1961).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000511842
0000511842
0000502649
0000502649
0000488804

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Mitsumasa Hashimoto et al.
The Journal of biological chemistry, 278(31), 28501-28507 (2003-05-16)
Closely opposed lesions form a unique class of DNA damage that is generated by ionizing radiation. Improper repair of closely opposed lesions could lead to the formation of double strand breaks that can result in increased lethality and mutagenesis. In
Differential scanning calorimetry of bacteria
Miles CA, et al.
Microbiology, 132(4), 939-952 (1986)
R Richins et al.
Biotechnology progress, 17(2), 252-257 (2001-04-21)
The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and
O Schröder et al.
Biochemical and biophysical research communications, 282(1), 219-227 (2001-03-27)
The DNA-binding protein H-NS compacts DNA and acts as a specific transcription factor regulating the expression of various bacterial genes. The small abundant protein binds to curved DNA without apparent sequence specificity and the exact nature of its DNA interaction
Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli
Hiller M, et al.
Chembiochem, 6(9), 1679-1684 (2005)
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