D2001

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Name: Deoxyribonucleic acid sodium salt from <I>Escherichia coli</I> strain B
Brand: SIGMA
Price: 1960 PLN

Type VIII

Synonim(y):

DNA

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5 MG

1960,00 zł

10 MG

3490,00 zł

25 MG

6920,00 zł

1960,00 zł

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Informacje o tej pozycji

Numer CAS:

93384-16-8

UNSPSC Code:

41106305

eCl@ss:

32160414

EC Number:

297-226-7

NACRES:

NA.51

MDL number:

MFCD00130922

Form:

fibers

Solubility:

water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow

Storage temp.:

2-8°C

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type

Type VIII

form

fibers

impurities

<2% protein

Quality Level

200

color

, white to white with yellow cast

solubility

water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow

λ_max

256-264 nm (15mM NaCl+1.5mM Trisodium Citrate PH7)

storage temp.

2-8°C

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Powiązane kategorie

Biochemicals

Nucleosides and Nucleotides

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Ta pozycja	D4889	D1501	D1626
Sigma-Aldrich D2001 Deoxyribonucleic acid sodium salt from Escherichia coli strain B	Sigma-Aldrich D4889 Deoxyribonucleic acid sodium salt from Escherichia coli strain B	Sigma-Aldrich D1501 Deoxyribonucleic acid sodium salt from calf thymus	Sigma-Aldrich D1626 Deoxyribonucleic acid sodium salt from salmon testes
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
form fibers	form lyophilized powder	form fibers	form solid
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
impurities <2% protein	impurities -	impurities -	impurities -
type Type VIII	type -	type Type I	type -

Application

Escherichia coli DNA has many uses in research. Escherichia coli DNA (ecDNA) is used in studies of DNA binding agents that modulate DNA structure and function. E. coli DNA is used in physicochemical studies of DNA behavior in solution, and to evaluate species-specific DNA structures and binding domains. This DNA is particularly useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols.

Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used to measure the melting temperature (Tm) and in differential scanning calorimetry (DSC) studies.[1] It has also been used as a template sequence in the polymerase chain reaction (PCR).[2]

Preparation Note

Prepared by procedure of Marmur, J., J. Mol. Biol., 3, 208 (1961).

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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

0000511842

0000502649

0000488804

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Odwiedź Bibliotekę dokumentów

Toward the three-dimensional structure of the Escherichia coli DNA-binding protein H-NS: A CD and fluorescence study.

O Schröder et al.

Biochemical and biophysical research communications, 282(1), 219-227 (2001-03-27)

The DNA-binding protein H-NS compacts DNA and acts as a specific transcription factor regulating the expression of various bacterial genes. The small abundant protein binds to curved DNA without apparent sequence specificity and the exact nature of its DNA interaction

Characterisation of cisplatin coordination sites in cellular Escherichia coli DNA-binding proteins by combined biphasic liquid chromatography and ESI tandem mass spectrometry.

Joanna Will et al.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 13(3), 421-434 (2007-12-25)

Combined multidimensional liquid chromatography and electrospray ionisation tandem mass spectrometry was employed to analyse platinated tryptic peptides from Escherichia coli cells treated with the anticancer drug cis-[PtCl2(NH3)2] at pH 7.0. Prerequisites for the LC/LC/MS/MS analysis of protein targets that are

Solid-state magic-angle spinning NMR of outer-membrane protein G from Escherichia coli

Hiller M, et al.

Chembiochem, 6(9), 1679-1684 (2005)

Effects of FIS overexpression on cell growth, rRNA synthesis, and ribosome content in Escherichia coli.

R Richins et al.

Biotechnology progress, 17(2), 252-257 (2001-04-21)

The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and

HU protein of Escherichia coli has a role in the repair of closely opposed lesions in DNA.

Mitsumasa Hashimoto et al.

The Journal of biological chemistry, 278(31), 28501-28507 (2003-05-16)

Closely opposed lesions form a unique class of DNA damage that is generated by ionizing radiation. Improper repair of closely opposed lesions could lead to the formation of double strand breaks that can result in increased lethality and mutagenesis. In

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Deoxyribonucleic acid sodium salt from Escherichia coli strain B