This product is intended for use as an attachment substrate for cell culture and has not been tested in western blotting as a positive control. However, the purification process of the starting material includes an affinity step and immunoelectrophoresis performed during Quality testing is against Anti-Fibronectin antibody F3648. While the end user would have to determine suitability, this material is expected to be suitable for use as a positive control in western blotting.
F0895
Human Fibronectin
from human plasma, liquid, 1 mg/mL, suitable for cell culture
FIBRONECTIN FROM HUMAN PLASMA
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Synonym(s):
Fibronectin
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mg | Check Cart for Availability | $316.00 | |
| 2 mg | Check Cart for Availability | $500.00 | |
| 5 mg | Check Cart for Availability | $1,080.00 |
About This Item
Biological source:
human plasma
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Concentration:
0.1% (Solution)
Product Name
FIBRONECTIN FROM HUMAN PLASMA, liquid, 0.1% (Solution), BioReagent, suitable for cell culture
biological source
human plasma
Quality Level
sterility
sterile
product line
BioReagent
form
liquid
packaging
pkg of 1 mg, pkg of 2 mg, pkg of 5 mg
concentration
0.1% (Solution)
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
solubility
saline: 0.05 M (Tris-buffered)
UniProt accession no.
binding specificity
Peptide Source: Collagen
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... FN1(2335)
General description
Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.
FN occurs as a dimeric protein, linked by two disulfide bonds at the end of C-terminal. Within the fibronectin structure, the domains are compactly arranged close to both the amino and carboxyl terminal of the peptide chains. Moreover, it has been observed that despite arising from a single gene up to 20 distinct variants of this gene have been identified in humans.
FN occurs as a dimeric protein, linked by two disulfide bonds at the end of C-terminal. Within the fibronectin structure, the domains are compactly arranged close to both the amino and carboxyl terminal of the peptide chains. Moreover, it has been observed that despite arising from a single gene up to 20 distinct variants of this gene have been identified in humans.
Application
Fibronectin from human plasma has been used in the determination of H2O2 consumption rate.[1] It has also been used in coating the Sylgard® models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.
Biochem/physiol Actions
Fibronectin function encompasses a diverse range of biological processes including cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Fibronectin protein plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Mutations in FN1 results in glomerulopathy.[2]
Features and Benefits
- Adhesive glycoprotein
- Derived from human plasma
- Recommended surface coverage of 1-5 μg/cm2
Preparation Note
This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C
Analysis Note
Protein homogeneity is evaluated by immunoelectrophoresis.
Legal Information
Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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This Item | |||
|---|---|---|---|
| biological source human plasma | biological source human fibroblasts | biological source human plasma | biological source human foreskin fibroblasts |
| sterility sterile | sterility sterile-filtered | sterility - | sterility - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| concentration 0.1% (Solution) | concentration ~0.5 mg/mL | concentration - | concentration - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form solution | form lyophilized powder | form lyophilized powder |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.
Articles
Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.
Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
Microcontact printing of fibronectin on a biodegradable polymeric surface for skeletal muscle cell orientation.
Altomare L, et al.
The International Journal of Artificial Organs, 33(8), 535-543 (2010)
Repression of KIAA1199 attenuates Wnt-signalling and decreases the proliferation of colon cancer cells.
Birkenkamp-Demtroder K, et al.
British Journal of Cancer, 105(4), 552-552 (2011)
The development of 3-D, in vitro, endothelial culture models for the study of coronary artery disease.
Farcas M A, et al.
BioMedical Engineering OnLine, 8(1), 30-30 (2009)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F0895-2MG | 04061833611487 |
| F0895-5MG | 04061833611494 |
| F0895-1MG | 04061833611470 |
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Could Fibronectin (F0895) be used as a positive control for fibronectin in western blot assay? If so, do one need to consider any extra steps in the preparation of such ctrl sample?
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How many µg/cm² would the coating concentration be if I applied a coating with a 0.1% concentration using the F0895 product?
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The concentration of the 0.1% solution can be found in the Certificate of Analysis. To calculate micrograms per square centimeter, consider the volume used and the surface area of the well.
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What is the recommended dilution rate for coating the culture surface with F0895-1MG Fibronectin as per the preparation instructions?
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The recommended dilution rate for coating the culture surface with F0895-1MG Fibronectin, as per the preparation instructions, is to start with a concentration in the range of 1 - 5 ug/cm^2. For a 96-well plate well with a surface area of about 0.34 cm^2, approximately 2 ul of F0895 further diluted in saline with a total volume of around 50 ul would be sufficient to cover the bottom of the well.
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What is the recommended storage duration for Fibronectin (F0895) at 4°C, -20°C, and -80°C? How long can a diluted solution of 10ug/mL in PBS be stored at 4°C? Is it possible to reuse the Fibronectin solution to coat culture ware surfaces multiple times?
1 answer-
The recommended storage duration for Fibronectin (F0895) is up to 1 year at 4°C. There has been no testing done on its stability after storage at -80°C or -20°C. A diluted solution of 10ug/mL in PBS can be stored at 4°C for 2-4 weeks. However, the solution cannot be reused to coat culture ware surfaces multiple times; the recommended process involves coating surfaces with a minimal volume, allowing them to air dry for 45 minutes at room temperature, and then storing the cultureware at 4°C for 2-4 weeks.
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Is Product F0895, Fibronectin from human plasma, sterile?
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Fibronectin 0.1% solution is a sterile product. It is tested for sterility. The sterility test results can be found on the lot-specific certificate of analysis.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should I store and handle Product F0895, Fibronectin from human plasma?
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Recommended storage of this material is 2-8 °C. Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
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What is the solvent for Product F0895, Fibronectin from human plasma?
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This material is solubilized in a 0.05 M Tris-buffered saline, pH 7.5.
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How much Product F0895, Fibronectin from human plasma, do I use for my application?
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Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
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