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F1141

Sigma-Aldrich

Fibronectin bovine plasma

solution, sterile-filtered, BioReagent, suitable for cell culture

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Synonym(s):
Fibronectin
EC Number:
MDL number:
eCl@ss:
42020141

biological source

bovine plasma

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

solution

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

UniProt accession no.

binding specificity

Peptide Source: Collagen

Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

2-8°C

Gene Information

bovine ... FN1(280794)

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1 of 4

This Item
F2006F1056F0287
vibrant-m

F1141

Fibronectin bovine plasma

vibrant-m

F2006

Fibronectin human plasma

vibrant-m

F1056

Fibronectin human plasma

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile

sterility

sterile

sterility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solution

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

General description

Fibronectin is a ubiquitous protein of the extracellular matrix. It is a mosaic protein, that is made up of three types of repeating modular consensus amino acid sequences. Fibronectins belongs to a class of high-molecular-weight glycoproteins. Cellular fibronectin is expressed by fibroblasts and plasma fibronectin is usually liberated by hepatocytes.

Application

Fibronectin (FN) bovine plasma has been used in:
  • fibronectin and adsorption analysis (ELISA (enzyme-linked immunosorbent assay))
  • transwell migration
  • fibronectin assay

Biochem/physiol Actions

Fibronectins participate in cell adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Other Notes

Product F1141, Fibronectin from bovine plasma is a solution in 0.05 M TBS pH 7.5.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Articles

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

See All

Protocols

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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