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Anti-Vimentin antibody produced in goat

Name: Anti-Vimentin antibody produced in goat
Brand: SIGMA
Price: 495 USD
Availability: InStock

whole antiserum

Synonym(s):

Anti-F5H288

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$495.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00145922

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IHC (f), IHC (p), WB

Citations:

$495.00

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Properties

biological source

goat

Quality Segment

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 58 kDa

contains

15 mM sodium azide

species reactivity

wide range, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:20 using formalin-fixed, paraffin-embedded sections of human tissue, western blot: 1:50 using HS-68 human foreskin cell extract

UniProt accession no.

P08670

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)

Description

General description

The VIM (vimentin) gene codes for an intermediate filament protein, that is expressed throughout mesenchymal cells. VIM gene is mapped to human chromosome 10p13.

Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain.

Immunogen

vimentin from cultured human foreskin fibroblasts.

Application

Anti-Vimentin antibody produced in goat has been used in Immunohistochemistry and Western blot analysis.

Anti-Vimentin antibody produced in goat has been used in following studies:

immunohistochemistry
western blotting
immunocytochemistry
flow cytometry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Biochem/physiol Actions

The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.

Vimentin is known to promote epithelial to mesenchymal transition and contributes to metastasis in cancer such as ovarian cancer. Vimentin preserves cellular structure and tissue integrity. Vimentin is also known to bring about TNF-α (tumor necrosis factor-α) induced cell apoptosis.

Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of the gene has been observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for treating these cancers. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1) induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments is implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. The protein interacts with LARP6 (la ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore mutations in the gene causes dominant and pulverulent cataract.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	V5255	V6389	SAB4503082
Sigma-Aldrich V4630 Anti-Vimentin antibody produced in goat	Sigma-Aldrich V5255 Monoclonal Anti-Vimentin antibody produced in mouse	Sigma-Aldrich V6389 Anti-Vimentin antibody, Mouse monoclonal	Sigma-Aldrich SAB4503082 Anti-Vimentin antibody produced in rabbit
description whole antiserum	description clone VIM-13.2, ascites fluid	description clone V9, purified from hybridoma cell culture	description affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
biological source goat	biological source mouse	biological source mouse	biological source rabbit
antibody form whole antiserum	antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in wet ice
species reactivity wide range, human	species reactivity chicken, rat, human, rabbit, frog, mouse	species reactivity hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human	species reactivity human

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Key Documents

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Anti-Vimentin antibody produced in goat

whole antiserum

Select a Size

About This Item

V4630-.2ML

$495.00

biological source

Quality Segment

conjugate

antibody form

antibody product type

clone

mol wt

contains

species reactivity

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Gene Information

General description

Immunogen

Application

Biochem/physiol Actions

Disclaimer

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Safety Information

Storage Class

wgk

flash_point_f

flash_point_c

ppe

Documentation

Certificates of Analysis (COA)

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