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The Renewable Electricity Supplier Toolkit helps suppliers learn renewable electricity basics and how to bring projects to life.

Viral Safety in Bioprocessing

Viral safety in bioprocessing relies on a framework of (1) preventing contamination, (2) detecting contamination, and (3) removing or inactivating viral contaminants.

Scalable Tangential Flow Filtration (TFF) for Downstream Processing of Viral Vectors for Gene Therapy and Vaccine Production

Our overview of scalable tangential flow filtration for viral vector production will provide key considerations for developing and optimizing your downstream process.

Membrane Prefilters Improve Sterilizing Filter Capacity and Process Economics in Bioprocessing

Prefilters can be used throughout bioprocessing to remove to remove particulates, improve quality and protect sterilizing filters and downstream unit operations.

Sterilizing Grade Filtration Unit Operations for Plasmid DNA Processes

We discuss the final filtration step of plasmid DNA manufacturing and its key considerations and challenges. Download our free pDNA downstream purification eBook or ask our experts.

Ultrafiltration/Diafiltration (UF/DF) in Plasmid DNA Manufacturing

How are ultrafiltration and diafiltration used in plasmid DNA manufacturing and how should you optimize this process? Learn more in our technical article and download our free eBook.

Tangential Flow Filtration in Downstream Bioprocessing

There are many options for tangential flow filtration (TFF) in downstream bioprocessing from multi-use to single-use TFF or batch TFF and single-pass TFF.

Water Determination in Liquefied Petroleum Gas using GC BID and Ionic Liquid Column Watercol™

Using Watercol™ GC columns in conjunction with GC-BID can provide a reliable and practical option for Water determination in petrochemical feedstocks.

Enzymatic Deglycosylation

Enzymatic Deglycosylation Strategies: Sequential hydrolysis of individual monosaccharides from glycans can be useful for the elucidation of the structure and function of the glycan component.

N-Glycosidase F Protocol & Troubleshooting

Neuraminidase (Sialidase) Protocol

Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.

Peptide Quick Tips

Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Tag Removal by Enzymatic Cleavage

This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.

Cell Disruption and Membrane Preparation

This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.

cOmplete™, EDTA-free Protocol

It is possible dissolve 1 tablet in 2 mL double dist. water (results in a 25x stock solution) without any problems.

cOmplete™, Mini, EDTA-free Protocol

Dissolve one tablet in 10 ml aqueous buffer or water. If high proteolytic activity is present, dissolve one tablet in 7 ml buffer.

Processing Small Tissue or Cell Samples using Sample Grinding Kit

Sample Grinding Kit from Cytiva is designed to disrupt cell or tissue samples for protein extraction.

Detergent-Free Nuclear Protein Extraction

Detergent-free procedure is recommended for nuclear protein preparation to avoid interference with labeling efficiency of extracted proteins.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

Liberase™ DH Research Grade Protocol & Troubleshooting

Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.

Liberase™ DL Research Grade Protocol & Troubleshooting

Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.

Liberase™ TH Protocol & Troubleshooting

Liberase Enzyme Blend selection depends on tissue dissociation protocol and success with collagenase.

Liberase™ TL Research Grade Protocol & Troubleshooting

Liberase™ TM Research Grade Protocol & Troubleshooting

Lysosome Isolation Kit

Lysosome Isolation Kit isolates lysosomes from tissues and cells for research purposes using differential centrifugation methods.

Protease Inhibition Using Protease Inhibitor Mix

Protease Inhibitor Mix from Cytiva inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.

TRI Reagent® Protocol

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

Antibody Drug Conjugate Mimic Enables LC-MS Method Development Without Risk

Here we show how LC and MS methods may be optimized using a non-toxic surrogate of the ADC, an “ADC-mimic”, that behaves very similarly to the Cys-linked ADC Adcetris (Seattle Genetics).

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