07-1719

Anti-FOXO3A Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, forkhead box O3, forkhead box O3A, forkhead homolog (rhabdomyosarcoma) like 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): mouse
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001446
• UniProt-Hinterlegungsnummer: O43524
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FOXO3(2309)

Beschreibung

Allgemeine Beschreibung

Forkhead box O3A (FOXO3A) is a member of the forkhead family of transcription factors (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. Upon oxidative stress, FOXO3A translocates to the nucleus where binding to the DNA sequence 5’-[AG]TAAA[TC]A-3’ occurs and IGF-1 is dephosphlorated, resulting in apoptosis. In order for the cell to survive, IGF-1 must be phosphlorated by AKT1/PKB at Thr32 and Ser-253, which will result in retention and inactivation of FOXO3A in the cytoplasm. Translocation of FOXO3A with the MLL gene has been associated with secondary acute leukemia.

~80 kDa

Immunogen

Synthetic peptide corresponding to FOX03A

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Insulin-/stoffwechselassoziierte Signalübertragung

Transkriptionsfaktoren

Immunocytochemistry:
A 1:500 dilution of this antibody was used to detect FOXO3A in HeLa cells. Positive staining in the nucleus.

This Anti-FOXO3A Antibody is validated for use in WB, IC for the detection of FOXO3A.

Biochem./physiol. Wirkung

This antibody recognizes FOXO3A.

Physikalische Form

Affinity purified

Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Angaben zur Herstellung

Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Hinweis zur Analyse

Control
FOXO3A in Jurkat cell lysate.

Western Blot Analysis:
1:500 dilution of this antibody was used to detect FOXO3A in Jurkat cell lysate.

Sonstige Hinweise

Replaces: 04-1007

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable