The reduction reagent used for this compound was sodium borohydride. The exact protocol used is proprietary to the lab and has not been disclosed, but the following publication may be of interest as a reference: Thomas G, Roques B., FEBS Lett. 1972; 26:169–175. The publication describes the process as follows: ...under nitrogen, in the dark and at 0°C, 500 mg of EB in 10 ml absolute methanol were added to 220 mg NaBH4. After 24 hr stirring, methanol was removed by evaporation in vacuum and 15 ml of 3% sodium hydroxide solution were added. After several extractions with ether, the phases were washed with water and dried over anhydrous sodium sulfate. Solvent was evaporated. The precipitate was recrystallized in ether to yield a light brown powder.
309800
Dihydroethidium
A cell-permeable, chemically-reduced ethidium derivative.
Synonym(e):
Dihydroethidium
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Qualitätsniveau
Beschreibung
RTECS - SF7935000
Assay
≥95% (HPLC)
Form
solid
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
protect from light
Farbe
pink
Löslichkeit
DMSO: 20 mg/mL
DMF: soluble
Versandbedingung
ambient
Lagertemp.
−20°C
SMILES String
Nc1c2c(cnc1)c3c(cc(cc3)N)C(C2CC)c4ccccc4
InChI
1S/C21H21N3/c1-2-15-20(13-6-4-3-5-7-13)17-10-14(22)8-9-16(17)18-11-24-12-19(23)21(15)18/h3-12,15,20H,2,22-23H2,1H3
InChIKey
ZDWPSSBKIMIPMY-UHFFFAOYSA-N
Verwandte Kategorien
1 of 4
Dieser Artikel | 37291 | D7008 | 309825 |
|---|---|---|---|
| color pink | color - | color pink to very dark red | color pink-white |
| form solid | form solid | form powder | form solid |
| solubility DMSO: 20 mg/mL, DMF: soluble | solubility acetonitrile: soluble, methanol: soluble | solubility chloroform: 20 mg/mL | solubility DMSO: 5 mg/mL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| assay ≥95% (HPLC) | assay ≥95% (HPCE) | assay ≥95% | assay ≥95% (HPLC) |
Allgemeine Beschreibung
Biochem./physiol. Wirkung
Nützliche fluorogene Sonde zum Nachweis reaktiver Sauerstoffspezies (ROS)
Verpackung
Angaben zur Herstellung
Sonstige Hinweise
Mancini, M., et al. 1997. J. Cell Biol.138, 449.
Carter, W.O., et al. 1994. J. Leukoc. Biol.55, 253.
Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
Perticarari, S., et al. 1991. Cytometry 12, 687.
Bucana, C.D., et al. 1990. Exp. Cell Res.190, 69.
Rothe, G., and Valet, G. 1990. J. Leukoc. Biol.47, 440.
Rechtliche Hinweise
Haftungsausschluss
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Analysenzertifikate (COA)
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I noticed that a compound from your catalogue is described as chemically reduced. I was wondering what the reducing agent used for this is?
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