5.30389

GSK′872

≥98% (HPLC), solid, RIPK3 inhibitor, Calbiochem^®

• Synonym(e): RIPK3 Inhibitor, GSK′872, N-(6-(Isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine, RIP3 Kinase Inhibitor I, Receptor-Interacting Protein Kinase 3 Inhibitor I, GSK-872, Necrosis Inhibitor V, RIPK3 Inhibitor I
• Empirische Formel (Hill-System): C₁₉H₁₇N₃O₂S₂
• CAS-Nummer: 1346546-69-7
• Molekulargewicht: 383.49
• MDL-Nummer: MFCD30481302
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Produktname: RIPK3 Inhibitor, GSK′872,
• Assay: ≥98% (HPLC)
• Qualitätsniveau: 100
• Form: solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: yellow
• Löslichkeit: DMSO: 100 mg/mL
• Lagertemp.: 2-8°C
• SMILES String: [S](=O)(=O)(C(C)C)c1cc2c([nH]cc[c]2=Nc3cc4nc[s]c4cc3)cc1
• InChI: 1S/C19H17N3O2S2/c1-12(2)26(23,24)14-4-5-16-15(10-14)17(7-8-20-16)22-13-3-6-19-18(9-13)21-11-25-19/h3-12H,1-2H3,(H,20,22)
• InChIKey: ZCDBTQNFAPKACC-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

A cell-permeable quinolinyl-benzothiazolamine compound that is reported to act as a RIP3-selective kinase inhibitor with >1,000-fold selectivity over a vast majority of more than 300 other kinases, including RIP1. RIP1 inhibitor Nec-1 (30 µM; Cat. Nos. 480065 & 505224 ) and genetic knockdown can be employed in conjunction with GSK′872 (3 µM) in delineating RIP1- vs. RIP3-dependency in Toll-like receptors- (TLRs) mediated necrosis and TNFR1-initiated necroptosis in the presence or absence of caspase inhibitor Z-VAD-FMK (25 µM; Cat. Nos. 627609 & 627610 ). While RIP3 and its downstream substrate MLKL are shown to mediate TLR3-initiated necrosis in the presence of Z-VAD-FMK in murine macrophage (BMDM & J774), fibroblast (3T3-SA & MEF), and endothelial (SVEC4-10) cultures, only in BMDM & J774 macrophage cultures is RIP1 involvement also seen in TLR3-mediated necrosis.

Biochem./physiol. Wirkung

Cell permeable: yes

Primary Target
RIP3 Kinase

Reversible: yes

Verpackung

Packaged under inert gas

Angaben zur Herstellung

Following reconstitution, aliquot and freeze 9-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sonstige Hinweise

Kaiser, W.J., et al. 2013. J. Biol. Chem.288, 31268.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable