UFC9010
Synonym(e):
10 kDa MWCO, 15 ml Probenvolumen, Zentrifugenkonzentrator
€ 151,00
€ 402,00
€ 1.530,00
Amicon® Ultra Zentrifugenfilter, 10 kDa MWCO, sample volume 15 mL, regenerated cellulose membrane, MWCO 10 kDa
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
non-sterile
Amicon®
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
Amicon® Ultra
Greener Alternative Product
15 mL sample volume
protein extraction: suitable
protein purification: suitable
12.1 cm (4.807 in.)
2.97 cm
7.6 cm2
15 mL
≤15 mL
15 mL
10 kDa MWCO
ambient
1 of 4
Dieser Artikel | UFC5010 | UFC8010 | UFC201024 |
|---|---|---|---|
| parameter 15 mL sample volume | parameter 0.5 mL sample volume | parameter 4 mL sample volume | parameter 2 mL sample volume |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Filtrationsmethoden und Membranfilterauswahl bei der Nanopartikelaufreinigung und -herstellung.
Auf dieser Seite werden die Grundsätze und Vorteile der Verwendung von Ultrafiltrationsmembranen bei der Vorbereitung von Proteinproben erörtert. Mögliche Anwendungen sind der Austausch von Lösungsmitteln, die Konzentration von Proteinproben oder die Probenaufreinigung.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Filtration methods and membrane filter selection in nanoparticle purification and production.
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
Proteinkonzentration ohne Verlust. Gewinnen Sie Proteine zuverlässig zurück, wenn Sie mit Amicon® Ultra Filtern konzentrieren.
The spin conditions for a swinging-bucker rotor are 4000 x g and for a fixed-angle rotor are 5000 x g, at a time range of 10 - 60 minutes, based on molecular weight cut-off.
Please see the link below to review the Product User Guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/181/pr05035wrev1023-ms.pdf
Helpful?
No data is available on the maximum or minimum temperature for using the Amicon Ultra-15. Results shown in the user guides are based on experiments conducted at approximately 25°C, not with refrigerated centrifuges. Some users work at 4°C, but filtration times are longer. No studies have been performed at 0°C. Glycerol is compatible with this product.
Helpful?
Concentrating cell-propagated respiratory syncytial virus stock with this product has not been studied. However, it can be attempted, as other viruses such as SARS, AAV, and Lenti have been successfully concentrated. The product concentrates substances larger than the molecular weight cut-off of the membrane. It is recommended to consult online research articles using keywords like "Amicon and respiratory syncytial virus" on platforms such as PubMed or Google Scholar.
Helpful?
Yes, product UFC5010, the Amicon Ultra 2.0 for samples of 0.5 - 2.0 mL, will accommodate a 1.5 mL sample size. Please see the link below to review this product option:
https://www.sigmaaldrich.com/product/mm/ufc5010
Helpful?
Does ultrafiltration of protein samples result in lower migration on an SDS-PAGE gel?
Yes, ultrafiltration of protein samples can potentially lead to lower migration on an SDS-PAGE gel.During the ultrafiltration process, the desalting or buffer exchange step may remove or reduce sodium chloride (NaCl), leading to lower protein migration on the gel. To prepare protein samples for gel electrophoresis, using an Amicon Ultra (AU) device or a suitable desalting/buffer exchange method is recommended.
Helpful?
Amicon® Ultra-15 Centrifugal Filter Unit comes in 8, 24, and 96/pk. the pack size is indicated in the last two digits of the catalog numbers.
Helpful?
This product is suitable for sample volumes of less than 15 mL. Please see the link below to review the estimated volumes:
https://www.sigmaaldrich.com/products/protein-biology/protein-sample-prep/amicon-centrifugals#howtoselect
Helpful?
We do not recommend reusing the device because eventually the membrane will clog completely. We have yet to record an efficient way to clean the pores of the membrane without damaging the membrane itself.
Helpful?
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