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α-Amylase aus Schweinepankreas

powder, ~50 U/mg

Synonym(e):

α-Amylase Enzyme

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Größe/SKUVerfügbarkeitPreis
25 g
Warenkorb auf Verfügbarkeit prüfen
€ 68,00
100 g
Warenkorb auf Verfügbarkeit prüfen
€ 188,00

Über diesen Artikel

UNSPSC Code:
12352204
EC Number:
232-565-6
NACRES:
NA.54
Specific activity:
~50 U/mg
Biological source:
hog pancreas

€ 68,00


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biological source

hog pancreas

Quality Segment

form

powder

specific activity

~50 U/mg

storage temp.

−20°C

General description

Pancreatic α-amylase is produced by the pancreatic acinar cells and released into the duodenum. It is a major molecule of pancreatic fluid.

Application

α-Amylase from hog pancreas has been used:
  • in the in vitro digestion system to study the hydrolysis of pea globulins
  • in vitro α-amylase inhibition study
  • to prepare digestive juice (salivary juice) for the in vitro model of the human digestion system

Biochem/physiol Actions

α-Amylase is a starch hydrolase, for glucose production, important for energy acquisition. It catalyzes the hydrolysis of α-1,4-glycosidic linkages in starch to form glucose, maltose, and maltotriose units. In mammals, α-amylase regulates the sugar assimilation by reducing the glucose uptake by Na+/glucose cotransporter 1 (SGLT1) and stimulating glycoprotein N-glycans mediated starch digestion. α-Amylase inhibitors are associated with the treatment of type 2 diabetes mellitus. α-Amylases are widely known industrial enzymes used in food, detergent, textile, fermentation, and pharmaceutical industries.

Other Notes

1 U entspricht der Enzymmenge, die 1 μmol Maltose pro Minute bei pH 6.9 und 25°C freisetzt (Stärke nach nach Zulkowsky, Fluka® Nr. 85642, als Substrat)
Optimum pH and temperature 6.9 and 53 °C, respectively; calcium is required for activity and also prevents degradation by trypsin; Characterization.

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Dieser Artikel
A3176A6255A4268
description

powder, ~50 U/mg

description

Type VI-B, ≥5 units/mg solid

description

PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)

description

Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

biological source

hog pancreas

biological source

Porcine pancreas

biological source

Porcine pancreas

biological source

Porcine pancreas

specific activity

~50 U/mg

specific activity

≥5 units/mg solid

specific activity

≥1000 units/mg protein (E1%/280)

specific activity

700-1400 units/mg protein (E1%/280)

form

powder

form

powder

form

saline suspension

form

saline suspension

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300


Piktogramme

Health hazard

Signalwort

Danger

Gefahrencodes

Sicherheitshinweise

Hazard Classifications

Resp. Sens. 1

Lagerklasse

11 - Combustible Solids

Was ist los?

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

PSA (Persönliche Schutzausrüstung)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Questions

  1. Is this Amylase contaminated with glucose? We do an experiment in which they digest a starch solution and denature the enzyme by pH in a sample then test using iodine and the benedicts reagent. If there is glucose contamination we'd get false positives.

    1 answer
    1. While this product is not expected to contain glucose, the material has not been tested for contaminating sugars.

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