A4268

α-Amylase aus Schweinepankreas

Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

• Synonym(e): β-N-Acetylglucosaminidase, Schweineplazenta, PPA, Schweinepankreas α-Amylase, al1,4-Glucan-4-glucanohydrolase,
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54
• MDL number: MFCD00081319
• EG-Nummer: 3.2.1.1 (BRENDA, IUBMB)
• Specific activity: 700-1400 units/mg protein (E1%/280)
• Biological source: Porcine pancreas

Eigenschaften

• biological source: Porcine pancreas
• Quality Segment: 300
• type: Type I-A
• form: saline suspension
• specific activity: 700-1400 units/mg protein (E1%/280)
• mol wt: 51-54 kDa
• sustainability: Sustainability features
• technique(s): activity assay: suitable
• suitability: suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification
• application(s): diagnostic assay manufacturing
• storage temp.: 2-8°C

Beschreibung

General description

Molekülmasse: 51–54 kDa.
α-Amylase, isoliert aus Schweine-Pankreas, ist ein Glykoprotein. Sie ist eine einzelne Polypeptidkette von ~475 Resten, die zwei SH-Gruppen und vier Disulfidbrücken sowie dicht gebundenes Ca²⁺ für die Stabilität enthält. Chloridionen sind notwendig für Aktivität und Stabilität. Der pH-Wertbereich für Aktivität beträgt 5,5 bis 8,0, wobei der optimale pH-Wert bei 7 liegt.

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Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis .

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca²⁺ necessary for stability.

Physical form

Suspension in 2.9 M NaCl-Lösung, enthält 3 mM CaCl_2.

Preparation Note

2x kristallisiert

Other Notes

1 U setzt 1.0 mg Maltose aus Stärke in 3 Minuten frei bei pH 6.9 und 20 °C.

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Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): dust mask type N95 (US), Eyeshields, Faceshields, Gloves