Fortfahren mit
P1243
pT7-FLAG™-2 Expression Vector
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
10 μg | Warenkorb auf Verfügbarkeit prüfen | € 412,00 |
Über diesen Artikel
NACRES:
NA.85
UNSPSC Code:
41106610
eCl@ss:
32150313
€ 412,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenrecombinant
expressed in E. coli
Quality Segment
grade
Molecular Biology
technique(s)
molecular cloning: suitable
shipped in
dry ice
storage temp.
−20°C
General description
pT7-FLAG-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.
The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
Vector Maps and Sequences
The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
Vector Maps and Sequences
Other Notes
- pT7-FLAG-2 Expression Vector, 10 μg (P6867) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pT7-FLAG-2-BAP Control Vector 1 μg ( P7117) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC
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Dieser Artikel | |||
|---|---|---|---|
| description - | description - | description - | description - |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| grade Molecular Biology | grade - | grade - | grade - |
| technique(s) molecular cloning: suitable | technique(s) molecular cloning: suitable | technique(s) - | technique(s) - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Lagerklasse
10 - Combustible liquids
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