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R0278

RIPA-Puffer

RIPA Buffer

Synonym(e):

Radioimmunoprecipitation assay buffer

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50 ML

€ 97,30

500 ML

€ 575,00

€ 97,30

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Über diesen Artikel

UNSPSC Code:
12161703
NACRES:
NA.56
MDL number:
MFCD02100484

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Unterstützung erhalten

description

protease free

form

liquid

packaging

pkg of 50 mL
pkg of 500 mL

technique(s)

immunoprecipitation (IP): suitable

color

colorless

pH

7.8-8.2 (25 °C, 1 ×)

storage temp.

2-8°C

Quality Level

200

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Dieser Artikel
20-18811814389001T9285
RIPA-Puffer

Sigma-Aldrich

R0278

RIPA-Puffer

RIPA-Lysepuffer, 10x 100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.

Millipore

20-188

RIPA-Lysepuffer, 10x

Lysepuffer für Erythrozyten solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions

Roche

11814389001

Lysepuffer für Erythrozyten

Tris-EDTA-Puffer -Lösung 100X concentrate for Northern and Southern blotting

Sigma-Aldrich

T9285

Tris-EDTA-Puffer -Lösung

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

200

form

liquid

form

liquid

form

solution

form

liquid

pH

7.8-8.2 (25 °C, 1 ×)

pH

-

pH

-

pH

7.8-8.2 (1 × in water)

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

room temp

color

colorless

color

-

color

-

color

-

Application

Der RIPA-Puffer ermöglicht eine effiziente Zelllyse und Solubilisierung von Proteinen und verhindert gleichzeitig den Proteinabbau und Interferenzen mit der Immunreaktivität und biologischen Aktivität der Proteine. Der RIPA-Puffer sorgt außerdem für einen niedrigen Hintergrund bei der Immunpräzipitation und bei molekularen Pulldown-Assays. Mit EZview™ Affinity Gelen kompatibel.
RIPA-Puffer wird in folgenden experimentellen Studien als Reagenz für die Zelllyse verwendet:
  • Fibroblastenbasierte präzise Phänotypisierung des peripheren Gewebes, wodurch die mechanistische Krankheitsschichtung in der sporadischen Parkinson-Erkrankung (sPD) unterstützt wird.[1]
  • Nachweis der Hemmung oxidativer Phosphorylierung (OXPHOS), welche die MLKL-abhängige Nekroptose in humanen Lungenepithelzellen aktivieren kann.[2]

Features and Benefits

1 mL RIPA-Puffer dient der Lyse von Zellen einer 100-mm-Kulturschale (0,5 bis 5 * 107 Zellen) der meisten adhärenten Säugerzelllinien.
Minimiert unspezifische Proteinbindungswechselwirkungen und gewährleistet somit einen niedrigen Hintergrund.

General description

Der Radioimmunpräzipitationspuffer (RIPA-Puffer) ist ein Lysepuffer, der eine schnelle, effiziente Zelllyse und Solubilisierung von Proteinen sowohl aus adhärenten als auch aus in Suspension kultivierten Säugerzellen ermöglicht.
Radio-Immunpräzipitationsassay(RIPA)-Puffer wird als gebrauchsfertige Lösung geliefert, die keine Zubereitung erfordert. Er vermindert unspezifische Proteinbindungsinteraktionen, um den Hintergrund zu minimieren, gewährt aber den Großteil an spezifischen Interaktionen, wodurch relevante Protein-Protein-Interaktionen untersucht werden können. Er wird häufig in Anwendungen wie beispielsweise der Immunpräzipitation eingesetzt, da die meisten Antikörper und Proteinantigene durch die Komponenten dieses Puffers nicht beeinträchtigt werden.

Other Notes

Gebrauchsfertige Lösung mit 150 mM NaCl, 1,0 % IGEPAL® CA-630, 0,5 % Natriumdesoxycholat, 0,1 % SDS, 50 mM Tris, pH 8,0.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC
IGEPAL is a registered trademark of Solvay

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000504725
0000504726
0000504726
0000504725
0000437799

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Die Dokumentenbibliothek aufrufen

Elham Abbasloo et al.
Neuropeptides, 60, 7-12 (2016-08-21)
Pain represents a major contributing factor to the individual's quality of life. Although pain killers as opioids, endogenous or exogenous peptides can decrease pain perception, the chronic use of them leads to antinociceptive tolerance. It has been demonstrated that neuropeptide
Impaired oxidative phosphorylation regulates necroptosis in human lung epithelial cells
Koo MJ, et al.
Biochemical and Biophysical Research Communications, 464(3), 875-880 (2015)
Paula Kinsella et al.
Investigational new drugs, 34(2), 159-167 (2016-02-18)
The C-KIT receptor tyrosine kinase is constitutively activated in the majority of gastrointestinal stromal tumours (GIST). Imatinib (IM) a selective inhibitor of C-KIT, is indicated for the treatment of KIT-positive unresectable and/or metastatic GIST, and has tripled the survival time
Zuzana Keckesova et al.
Nature, 543(7647), 681-686 (2017-03-23)
Post-mitotic, differentiated cells exhibit a variety of characteristics that contrast with those of actively growing neoplastic cells, such as the expression of cell-cycle inhibitors and differentiation factors. We hypothesized that the gene expression profiles of these differentiated cells could reveal
Hartmut Jahns et al.
Nature communications, 6, 6317-6317 (2015-03-07)
An established means of improving the pharmacokinetics properties of oligoribonucleotides (ORNs) is to exchange their phosphodiester linkages for phosphorothioates (PSs). However, this strategy has not been pursued for small interfering RNAs (siRNAs), possibly because of sporadic reports that PS siRNAs
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Product Information Sheet - R0278

Instructions

GC5, GC10, and Thunderbolt Competent Cells
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Questions

1–10 of 12 Questions  

  1. we bought a bottle of RIPA buffer and want to make alliquots of this bottle. Can we store the alliquots of the buffer by -20? Or is it better to keep them between 2°C-8°C ?

    1 answer

    1. It is recommended to store the product at 2–8C. Studies have not been performed on aliquots and to determine if refrigerated storage will extend the shelf life of this product. We would recommend searching the general research literature or contacting researchers who routinely use this product to see if solution stability information is available. If a recommended storage is not given in the literature, it is usually best to make solutions fresh for use. If you decide to store the aliquots at -20°C, we would recommend validating activity of the supplemented buffer after freezing and avoid any thawing/freezing cycle. Please refer to this technical bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  2. Is this RIPA lysis buffer capable of extracting nuclear protein in mammalian cells?

    1 answer

    1. Yes, RIPA buffer typically lyses cell nuclei. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  3. Can I use R0278 for human protein extract for lymphocytes?

    1 answer

    1. This product has not been tested for protein extract from human lymphocytes. References using RIPA buffer with human lymphocytes can be found here:
      https://www.sciencedirect.com/science/article/pii/S0021925818639675
      https://www.sciencedirect.com/science/article/pii/S000887491530037X?via%3Dihub

      Helpful?

  4. Can I use the Ripa Buffer to extract yeast protein and make the Bradford assay on it?

    1 answer

    1. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  5. How long can the RIPA buffer be stored in 4C? I stored it for almost 6 years, can I still use it?

    1 answer

    1. Regarding stability in the recommended temperature of 2-8 °C, this product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  6. Can this be used for Western blot experiments?

    1 answer

    1. Yes, this product would be suitable for western blotting.

      Helpful?

  7. Can Product R0278, RIPA buffer, be used on tissue samples, or only on cultured cells?

    1 answer

    1. R0278 can be used on tissue, if it is well homogenized.

      Helpful?

  8. How does Product R0278, RIPA buffer, compare to Product No. C2978 (CelLytic M)?

    1 answer

    1. RIPA buffer contains SDS, which is considered to be a harsh detergent; it can denature many proteins. C2978 is a buffered solution (pH 7.6) that contains a mild detergent; it is non-denaturing for most proteins.  The exact formulation of C2978, however, is proprietary.

      Helpful?

  9. I left the bottle of Product R0278, RIPA buffer, on my bench for about a week. Can I still use it?

    1 answer

    1. Yes, you should be able to use this product.  Although we recommend storage in the refrigerator at 2-8°C for long-term storage, Product No. R0278 may be kept at ambient temperature (our normal shipping condition) for a short time (approximately 2 weeks).

      Helpful?

  10. Does Product R0278, RIPA buffer, contain any protease inhibitors or phosphatase inhibitors?

    1 answer

    1. This buffer does not contain any protease or phosphatase inhibitors, however, they may be added to insure against possible proteolysis.

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