Metabolic profiling of Vitex agnus castus leaves, fruits and sprouts: analysis by LC/ESI/(QqQ)MS and (HR) LC/ESI/(Orbitrap)/MS n.

Metabolic profiling of Vitex agnus castus leaves, fruits and sprouts: analysis by LC/ESI/(QqQ)MS and (HR) LC/ESI/(Orbitrap)/MS n.

Journal of pharmaceutical and biomedical analysis (2014-10-12)

Angela Mari, Paola Montoro, Gilda D'Urso, Mario Macchia, Cosimo Pizza, Sonia Piacente

ZUSAMMENFASSUNG

Food supplements based on Vitex agnus castus L. (Verbenaceae) fruits, also known as chasteberry, are routinely used by women against somatic and psychic premenstrual symptoms such as depression, sadness or irritability. With the aim of highlighting the differences in the chemical profiles of cultivated fruits and different parts of wild plants (fruits, leaves and sprouts) of V. agnus castus, a method concerning with the quali-quantitative study of the derived hydroalcoholic extracts was carried out by using high-performance liquid chromatography coupled to electrospray negative ionization Orbitrap multicollisional high resolution mass spectrometry (LC/ESI/(Orbitrap)MS(n)) and high-performance liquid chromatography coupled to electrospray negative ionization triple quadrupole tandem mass spectrometry (LC/ESI/(QqQ)MS) in multiple reaction monitoring (MRM) mode.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

34860

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Methanol, suitable for HPLC, ≥99.9%

E7023

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Ameisensäure, reagent grade, ≥95%

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Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer

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Ethyl alcohol, Pure, 200 proof, meets USP testing specifications

319988

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Chloroform, contains ethanol as stabilizer, ACS reagent, ≥99.8%

366927

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Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

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Ameisensäure, ACS reagent, ≥96%

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Ethanol, BioUltra, Molecular Biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)

320390

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USP

Methylalkohol, United States Pharmacopeia (USP) Reference Standard