Polymer enzyme conjugates as chiral ligands for sharpless dihydroxylation of alkenes in organic solvents.

Polymer enzyme conjugates as chiral ligands for sharpless dihydroxylation of alkenes in organic solvents.

Chembiochem : a European journal of chemical biology (2014-11-26)

Stefan Konieczny, Melanie Leurs, Joerg C Tiller

ZUSAMMENFASSUNG

Conjugates of enzymes and poly(2-methyloxazoline) were used as organosoluble amphiphilic polymer nanocontainers for dissolving osmate, thereby converting the enzymes into organosoluble artificial metalloenzymes. These were shown to catalyze the dihydroxylation of different alkenes with high enantioselectivity. The highest selectivities, found for osmate complexed with laccase polymer-enzyme conjugates (PECs), even exceed those of classical Sharpless catalysts.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

34860

Sigma-Aldrich

Methanol, suitable for HPLC, ≥99.9%

E7023

Sigma-Aldrich

Ethyl alcohol, Pure 200 proof, Molecular Biology

179337

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

459844

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%

673811

Sigma-Aldrich

Diethylether, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor

C2432

Sigma-Aldrich

Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%

646377

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%

650498

Sigma-Aldrich

Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer

493546

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, meets USP testing specifications

309966

Sigma-Aldrich

Diethylether, suitable for HPLC, ≥99.9%, inhibitor-free

319988

Sigma-Aldrich

Chloroform, contains ethanol as stabilizer, ACS reagent, ≥99.8%

366927

Sigma-Aldrich

Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

346136

Sigma-Aldrich

Diethylether, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor

459836

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

322415

Sigma-Aldrich

Methanol, anhydrous, 99.8%

E7148

Sigma-Aldrich

Ethyl alcohol, Pure 190 proof, for molecular biology

179957

Sigma-Aldrich

Methanol, Laboratory Reagent, ≥99.6%

439193

Sigma-Aldrich

Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%

493511

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

154903

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

472476

Sigma-Aldrich

Chloroform, contains amylenes as stabilizer, ACS reagent, ≥99.8%

51976

Sigma-Aldrich

Ethanol, BioUltra, Molecular Biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)

320390

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

650471

Sigma-Aldrich

Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 0.5-1.0% ethanol as stabilizer

296082

Sigma-Aldrich

Diethylether, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%

B103500

Sigma-Aldrich

Buttersäure, ≥99%

P0381

Sigma-Aldrich

Pyrogallol, ≥98% (HPLC)

366919

Sigma-Aldrich

Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

M1775

Sigma-Aldrich

Methanol, Absolute - Acetone free

132950

Sigma-Aldrich

Chloroform, ReagentPlus^®, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer