A New Organic Dye-Based Staining for The Detection of Plant DNA in Agarose Gels.

A New Organic Dye-Based Staining for The Detection of Plant DNA in Agarose Gels.

Nucleosides, nucleotides & nucleic acids (2015-07-15)

Özlem Ateş Sönmezoğlu, Kerime Özkay

ZUSAMMENFASSUNG

Ethidium bromide (EtBr) is used to stain DNA in agarose gel electrophoresis, but this dye is mutagenic and carcinogenic. We investigated N-719, which is a visible, reliable and organic Ruthenium-based dye, and five fluorescent alternatives for staining plant DNA. For prestaining and poststaining, N-719, GelRed, and SYBR Safe stained both DNA and PCR product bands as clearly as EtBr. SYBR Green I, methylene blue, and crystal violet were effective for poststaining only. The organic dye N-719 stained DNA bands as sensitively and as clearly as EtBr. Consequently, organic dyes can be used as alternatives to EtBr in plant biotechnology studies.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

D2650

Sigma-Aldrich

Dimethylsulfoxid, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

D8418

Sigma-Aldrich

Dimethylsulfoxid, Molecular Biology

D2438

Sigma-Aldrich

Dimethylsulfoxid, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

276855

Sigma-Aldrich

Dimethylsulfoxid, anhydrous, ≥99.9%

D4540

Sigma-Aldrich

Dimethylsulfoxid, ≥99.5% (GC), suitable for plant cell culture

M1028

Sigma-Aldrich

Magnesiumchlorid -Lösung, Molecular Biology, 1.00 M±0.01 M

M8266

Sigma-Aldrich

Magnesiumchlorid, ≥98%

E1510

Sigma-Aldrich

Ethidiumbromid -Lösung, BioReagent, Molecular Biology, 10 mg/mL in H₂O

41639

Sigma-Aldrich

Dimethylsulfoxid, BioUltra, Molecular Biology, ≥99.5% (GC)

M9140

Sigma-Aldrich

Methylenblau, certified by the BSC

208337

Sigma-Aldrich

Magnesiumchlorid, powder, <200 μm

03978

Sigma-Aldrich

Methylenblau -Lösung, concentrate according to Ehrlich, concentrated, aqueous solution

D1435

Sigma-Aldrich

Dimethylsulfoxid, meets EP testing specifications, meets USP testing specifications

68475

Sigma-Aldrich

Magnesiumchlorid -Lösung, BioUltra, Molecular Biology, 2 M in H₂O

63069

Sigma-Aldrich

Magnesiumchlorid -Lösung, BioUltra, Molecular Biology, ~1 M in H₂O

E7637

Sigma-Aldrich

Ethidiumbromid, BioReagent, Molecular Biology, powder

E1385

Sigma-Aldrich

Ethidiumbromid -Lösung, BioReagent, Molecular Biology, 500 μg/mL in H₂O

D9170

Sigma-Aldrich

Dimethylsulfoxid, PCR Reagent

M4880

Sigma-Aldrich

Magnesiumchlorid, suitable for insect cell culture, BioReagent, ≥97.0%

206180

Sigma-Aldrich

Ruthenium auf Kohlenstoff, extent of labeling: 5 wt. % loading

M8787

Sigma-Aldrich

Magnesiumchlorid -Lösung, PCR Reagent, 25 mM MgCI₂solution for PCR

319112

Sigma-Aldrich

Methylenblau -Lösung, 0.05 wt. % in H₂O

E8751

Sigma-Aldrich

Ethidiumbromid, ~95% (HPLC)

545023

Sigma-Aldrich

Ruthenium, powder, 99.99% trace metals basis

449164

Sigma-Aldrich

Magnesiumchlorid, AnhydroBeads^™, −10 mesh, 99.9% trace metals basis

46067

Sigma-Aldrich

Ethidiumbromid -Lösung, suitable for fluorescence, ~1% in H₂O

449172

Sigma-Aldrich

Magnesiumchlorid, AnhydroBeads^™, −10 mesh, 99.99% trace metals basis

46047

Supelco

Pyridaben, PESTANAL^®, analytical standard

1808

Sigma-Aldrich

Methylenblaulösung 1,4 % (w/v) 95 % Ethanol | 7220-79-3, 1.4 % (w/v) in 95% ethanol

209694

Sigma-Aldrich

Ruthenium, powder, −200 mesh, 99.9% trace metals basis