- Experience-dependent DNA methylation regulates plasticity in the developing visual cortex.
Experience-dependent DNA methylation regulates plasticity in the developing visual cortex.
Nature neuroscience (2015-05-26)
Paola Tognini, Debora Napoli, Jonida Tola, Davide Silingardi, Floriana Della Ragione, Maurizio D'Esposito, Tommaso Pizzorusso
PMID26005848
ZUSAMMENFASSUNG
DNA methylation is an epigenetic repressor mark for transcription dynamically regulated in neurons. We analyzed visual experience regulation of DNA methylation in mice and its involvement in ocular dominance plasticity of the developing visual cortex. Monocular deprivation modulated the expression of factors controlling DNA methylation and exerted opposite effects on DNA methylation and hydroxymethylation in specific plasticity genes. Inhibition of DNA methyltrasferase (DNMT) blocked molecular and functional effects of monocular deprivation, partially reversing the monocular deprivation transcriptional program.
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Natriumdodecylsulfat, BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC)
Sigma-Aldrich
Phenol -Lösung, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, Molecular Biology
Sigma-Aldrich
Phenol:Chloroform:Isoamylalkohol 25:24:1, gesättigt mit 100 mM TRIS pH 8,0; 1 mM EDTA, Supplied with Equilibration buffer, Molecular Biology
Sigma-Aldrich
Ethylendiamintetraessigsäure, anhydrous, crystalline, BioReagent, suitable for cell culture
Sigma-Aldrich
Phenol, puriss., ≥99.5% (GC), meets analytical specification of Ph. Eur., BP, USP, crystalline (detached)
Sigma-Aldrich
Ethylendiamintetraessigsäure -Lösung, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
Supelco
Natriumdodecylsulfat, dust-free pellets, suitable for electrophoresis, Molecular Biology, ≥99.0% (GC)
Sigma-Aldrich
Phenol -Lösung, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology