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Merck

05-678

Anti-ubiquityl-Histone H2A Antibody, clone E6C5

clone E6C5, Upstate®, from mouse

Sinônimo(s):

H2AUb, Histone H2A (ubiquityl), H2A histone family, member R, histone 1, H2aa, histone H2A, histone cluster 1, H2aa, H2AK119Ub1

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Sobre este item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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Nome do produto

Anti-ubiquityl-Histone H2A Antibody, clone E6C5, clone E6C5, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

E6C5, monoclonal

species reactivity

amphibian, mouse, human, frog, rat, monkey

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgM

NCBI accession no.

NP_003508

UniProt accession no.

Q96QV6

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

frog ... H2Ac1(594915)
human ... H2AC1(221613)
mouse ... H2Ac1(319163)
rat ... H2Ac1(24828)

Analysis Note

Control
Acid extracts of HeLa cells.
Routinely evaluated by western blot on H2A in acid extracts from HeLa and 10T1/2 cells.

Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected ubiquityl-Histone H2A in acid extracts from HeLa and 10T1/2 cells.

Application

Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect ubiquityl-Histone H2A in cells fixed with either 3% formaldehyde/0.1% Triton X-100 or methanol (Vassilev, A. 1995).

Chromatin Immunoprecipitation:
Reported by an independent laboratory (Wang, H. 2004).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-ubiquityl-Histone H2A Antibody, clone E6C5 (Mouse Monoclonal Antibody) has been published and validated for use in ChIP, ICC, WB to detect ubiquityl-Histone H2A also known as H2AUb, Histone H2A (ubiquityl), histone H2A.

Biochem/physiol Actions

Does not cross-react with army worm.
Other species not tested.
This antibody recognizes and is specific for monoubiquityl-Histone H2A (Lys119), Mr ~25 kDa.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

25 kDa
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Ubiquitylation usually targets the substrate for degradation, although histones H2A and H2B are actually stabilized by a single ubiquitin conjugation. Histone ubiquitination has been correlated with DNA repair and transcription, cellular differentiation, cell cycle regulation, spermatogenesis, protein trafficking, and response to stress. Histone H2A is monoubiquitinated at Lys119 by the PRC-1L complex (Polycomb repressive complex 1-like), which includes Ring1, Ring2, Bmi1 and HPH2. Ubiquitinated H2A normally represents about 15% of H2A, but this value can be as high as 50% in active chromatin. Ubiquitinyl histone H2A has also been associated with transcriptional silencing of large chromatin regions and linked to Polycomb silencing so the function of ubiquitinated H2A remains undefined.

Immunogen

AMA (human epithelial amnion) cell residual nuclear pellet portions.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Purified mouse monoclonal IgM in buffer containing PBS with 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC.

Preparation Note

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de armazenamento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Qin Li et al.
Carcinogenesis, 30(7), 1243-1251 (2009-04-21)
In the present study, we examined the effects of CoCl(2) on multiple histone modifications at the global level. We found that in both human lung carcinoma A549 cells and human bronchial epithelial Beas-2B cells, exposure to CoCl(2) (>/=200 muM) for
Genome-wide uH2A localization analysis highlights Bmi1-dependent deposition of the mark at repressed genes.
Kallin, EM; Cao, R; Jothi, R; Xia, K; Cui, K; Zhao, K; Zhang, Y
PLoS Genetics null
Yanlai Lai et al.
Archives of biochemistry and biophysics, 518(2), 103-110 (2012-01-12)
Inactivation of the von Hippel-Lindau (VHL) tumor suppressor is associated with renal carcinoma, hemangioblastoma and pheochromocytoma. The VHL protein is a component of a ubiquitin ligase complex that ubiquitinates and degrades hypoxia inducible factor-α (HIF-α). Degradation of HIF-α by VHL
Wei-Ting Liao et al.
Carcinogenesis, 30(6), 1064-1072 (2009-04-21)
Bowen's disease (BD), a carcinoma in situ of the skin, has been identified as an early lesion in arsenic carcinogenesis. Patients with arsenic-induced Bowen's disease (As-BD) showed both cutaneous and systemic immune dysfunctions. We set out to evaluate the interactions
dKDM2 couples histone H2A ubiquitylation to histone H3 demethylation during Polycomb group silencing.
Lagarou, A; Mohd-Sarip, A; Moshkin, YM; Chalkley, GE; Bezstarosti, K; Demmers, JA; Verrijzer, CP
Genes & Development null
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