MAB4037
Anti-PTEN Antibody, CT, clone A2b1
clone A2b1, Chemicon®, from mouse
Sinônimo(s):
MMAC1, TEP1
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Sobre este item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Estamos aqui para ajudarfonte biológica
mouse
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
A2b1, monoclonal
reatividade de espécies
human, rat, mouse
fabricante/nome comercial
Chemicon®
técnica(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG1
adequação
not suitable for immunohistochemistry (Paraffin)
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... PTEN(5728), TEP1(7011)
Descrição geral
55 kDa
Imunogênio
Anti-PTEN was generated by immunization with a 10 amino acid peptide corresponding to amino acids 387-400 near the C-terminus of human PTEN.
Epitope: C-terminus
Aplicação
Anti-PTEN Antibody, C-terminus, clone A2b1 is an antibody against PTEN for use in IC, IH, IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Western blot 1:100 (55 kDa)
Immunocytochemistry
Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.
Immunoprecipitation 1:10 to 1:20
Protocol for immunohistochemistry of frozen tissue sections on glass slides.
1. Fix sections for 5 minutes with cold acetone.
2. Air dry
3. Wash sections with PBS
4. Add blocking solution (horse serum) for 20 minutes
5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.
6. Wash with PBS
7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).
8. Incubate for 30 minutes at room temperature.
9. Wash with PBS
10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.
11. Wash with PBS
12. Develop with DAB substrate.
13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.
Immunoprecipitation 1:10 to 1:20
Protocol for immunohistochemistry of frozen tissue sections on glass slides.
1. Fix sections for 5 minutes with cold acetone.
2. Air dry
3. Wash sections with PBS
4. Add blocking solution (horse serum) for 20 minutes
5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.
6. Wash with PBS
7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).
8. Incubate for 30 minutes at room temperature.
9. Wash with PBS
10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.
11. Wash with PBS
12. Develop with DAB substrate.
13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.
Ações bioquímicas/fisiológicas
Specifically recognizes PTEN*, a tumor suppressor protein located on human chromosome 10 (Steck, ′97; Li, ′97; Rhei, ′97) that is often mutated in various types of advanced cancers (Rhei, ′97). The PTEN protein exhibits protein phosphatase activity (Myers, ′97) and can suppress the growth of glioma cells (Furnari, ′97). The antibody recognizes human and mouse PTEN and is specific for the C-terminus. Other species not tested. Reactivity with human fibroblasts, breast epithelial and lung cancer cell lines has been confirmed by western blot, and antibody specificity has been confirmed by peptide blocking studies.
*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.
*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.
forma física
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Nota de preparo
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análise
Control
Breast tumor tissue
Purified active kinase is Catalogue Number 14-488
Breast tumor tissue
Purified active kinase is Catalogue Number 14-488
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-409
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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