CLLS1069

MCF10A CELLS APC (-/-)

human female mammary glands (Source Disease: Fibrocystic Disease)

• UNSPSC Code: 41106514
• eCl@ss: 32190102
• Biological source: human female mammary glands (Source Disease: Fibrocystic Disease)

Propriedades

• Nome do produto: MCF10A CELLS APC (-/-),
• biological source: human female mammary glands (Source Disease: Fibrocystic Disease)
• Quality Segment: 200
• OMIM accession no.: 611731
• storage temp.: −196°C
• Gene Information: human ... APC(324)

Descrição

General description

MCF10A CELLS APC (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.
This cell line corresponds to ATCC Catalog No. CRL-10317.
Adenomatous polyposis coli (APC) is a tumor suppressor gene. It is located on human chromosome 5q21.

Application

Cell freezing medium-DMSO 1X, Cat. No. C6164

Media renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°

The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

Biochem/physiol Actions

Adenomatous polyposis coli (APC) plays an important role in cell division, adhesion and migration. Mutations in APC gene is associated with colorectal carcinomas.

Features and Benefits

These MCF10A cells are adherent with a doubling time of approx. 20 hours

Zinc finger nuclease (ZFN) knockout on chromosome 5q21

Analysis Note

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Informações de segurança

• Classe de armazenamento: 12 - Non Combustible Liquids
• WGK: WGK 3
• Ponto de inflamação (°F): Not applicable
• Ponto de inflamação (°C): Not applicable