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Merck

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D6429

DMEM - high glucose

With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

2 of 6 reviewers received a sample product or took part in a promotion

Sinônimo(s):

DME, DMEM

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UNSPSC Code:
12352207
NACRES:
NA.71
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Nome do produto

Meio de Eagle modificado por Dulbecco - alto teor de glicose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

General description

Este meio de glicose DMEM-Hi é um meio completo concentrado 1x, com adição de piruvato de sódio. Ele também difere da formulação original do DMEM-Hi em que a piridoxina é substituída por piridoxal. O piridoxal é um componente instável do meio.

Application

Meio de Eagle modificado por Dulbecco - usou-se alto teor de glicose para cultura celular.
O meio de Eagle modificado por Dulbecco (DMEM) é uma modificação do meio basal Eagle (BME) que contém concentrações quatro vezes maiores de aminoácidos e vitaminas. A formulação original contém 1000 mg/l de glicose e foi usada para cultura de células embrionárias de camundongo. Desde então, ele foi modificado de várias maneiras para dar suporte a culturas primárias de células de camundongo e frango, além de diversas células normais e transformadas. Cada um desses meios oferece uma combinação diferente de L-glutamina e piruvato de sódio. Além disso, os níveis de glicose foram elevados para 4500 mg/l, contribuindo para o nome "DMEM/alto".

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Este Item
FG0445D7777D5648
description

With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

description

With 4500 mg/L glucose, L-Ala-L-Gln, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

description

With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

description

With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

powder

form

powder

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

components

NaHCO3: yes
stable glutamine: yes
glucose: high
phenol red: yes
HEPES: no
sodium pyruvate: yes

components

glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes

components

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient


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12 - Non Combustible Liquids



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Certificados de análise (COA)

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Questions

1–5 of 5 Questions  
  1. How much does D6429-1L weigh? Thank you!

    1 answer
    1. This product is a liquid medium. The density is not reported. The 1L volume weighs approximately 1 kilogram. The total weight of the packaged 1L size is 1.1 kg. The total weight of the packaged 500 mL size is 578 g.

      Helpful?

  2. Hi, What is the concentration of endotoxin detected in DMEM high glucose media D6429?

    1 answer
    1. This product has an endotoxin specification of ≤ 1 EU/ml or 0.1 ng/mL. The lot specific values are reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/d6429#product-documentation

      Helpful?

  3. What is the concentration of Sodium Bicarbonate and Sodium Pyruvate in it?

    1 answer
    1. This formulation contains 3.7 g/L sodium bicarbonate and 110 mg/L sodium pyruvate. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf

      Helpful?

  4. After opening a bottle of medium: how long can I use this bottle ?(stored at 4C°)

    1 answer
    1. Provided that the product is handled and stored properly and has not been contaminated, the medium will be stable up to the expiration date. This date is provided on the label and the lot-specific Certificate of Analysis.

      Helpful?

  5. I would like to inquire about the amino acid formulation in DMEM (#6429, #6546). Specifically, could you provide information on the concentration of aspartate and glutamate in these formulations? Thank you very much.

    1 answer
    1. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf

      Helpful?

Reviews

Active Filters

  1. Atlanta, GA
    • Reviews 4
    • Votes 107
    5 out of 5 stars.

    D6171 with HEPES modification

    The best media to culture HEK293FT cells. The HEPES modification seems beneficial during calcium-phosphate transfection by perhaps stabilizing pH.

    Helpful?

  2. 5 Ratings-only reviews