OGS3164

PSF-OXB20-NH2-TORA - TORA SECRETION PLASMID

plasmid vector for molecular cloning

• Sinônimo(s): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• UNSPSC Code: 12352200
• eCl@ss: 32160703
• Promoter: Promoter name: OXB20; Promoter activity: constitutive; Promoter type: bacterial
• Origin of replication: pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: none
• Peptide cleavage: no cleavage

Propriedades

• recombinant: expressed in E. coli
• form: buffered aqueous solution
• mol wt: size 3965 bp
• technique(s): molecular cloning: suitable
• bacteria selection: kanamycin
• origin of replication: pUC (500 copies)
• peptide cleavage: no cleavage
• peptide tag location: N-terminal
• promoter: Promoter name: OXB20; Promoter activity: constitutive; Promoter type: bacterial
• reporter gene: none
• secretion signal: TorA
• shipped in: ambient
• storage temp.: −20°C

Descrição

General description

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains an TorA secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. .

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Other Notes

To view sequence information for this product, please visit the product page

Informações de segurança

• Classe de armazenamento: 12 - Non Combustible Liquids
• Ponto de inflamação (°F): Not applicable
• Ponto de inflamação (°C): Not applicable