OGS3441

PSF-TEF1-COOH-EKT-6HIS - C-TERMINAL 6 HIS TAG YEAST PLASMID

plasmid vector for molecular cloning

• Sinônimo(s): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• UNSPSC Code: 12352200
• Promoter: Promoter name: TEF1; Promoter activity: constitutive; Promoter type: yeast
• Origin of replication: 2Micron, pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: none
• Peptide cleavage: EKT

Propriedades

• tag: 6-His tagged
• form: buffered aqueous solution
• mol wt: size 6750 bp
• bacteria selection: kanamycin
• origin of replication: 2Micron, pUC (500 copies)
• peptide cleavage: EKT
• peptide tag location: C-terminal
• promoter: Promoter name: TEF1; Promoter activity: constitutive; Promoter type: yeast
• reporter gene: none
• shipped in: ambient
• storage temp.: −20°C
• yeast selection: uracil

Descrição

General description

This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.

About the Peptide Tag:This plasmid contains a c-terminal Hexa-Histidine (6His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHH.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.

Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

Other Notes

Quick-reference Plasmid Map

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics^™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Genebank Vector Sequence File

FASTA Vector Sequence File

Full Plasmid Map

Legal Information

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Informações de segurança

• Classe de armazenamento: 12 - Non Combustible Liquids
• Ponto de inflamação (°F): Not applicable
• Ponto de inflamação (°C): Not applicable