Passa al contenuto
Merck

07-131-AF488

Anti-Sirt1 (Sir2) Antibody, Alexa Fluor™ 488 Conjugate

from rabbit, ALEXA FLUOR™ 488

Sinonimo/i:

NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2alpha, Sir2, mSIR2a, SirtT1 75 kDa fragment, 75SirT1, Sirt1(Sir2), Alexa Fluor™ 488 Conjugate

Autenticati per visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

Informazioni su questo articolo

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.45

Documenti fondamentali

Visualizza tutta la documentazione
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutarti
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutarti

Origine biologica

rabbit

Livello qualitativo

100

Coniugato

ALEXA FLUOR™ 488

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

mouse, human

tecniche

immunocytochemistry: suitable

N° accesso NCBI

NP_062786

N° accesso UniProt

Q923E4

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... SIRT1(23411)

Descrizione generale

NAD-dependent protein deacetylase sirtuin-1 (UniProt Q923E4; also known as Sir2, mSIRT2a, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, Sir2-like 1, SIR2-like protein 1, SIR2alpha) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID 93759) in murine species. Sirtuins constitute a family of NAD+-dependent deacetylases found in nearly all organisms. Sirtuins have been implicated in the regulation of aging, transcription, apoptosis, and stress resistance. Full-length SirT1 (FLSirT1) plays an important role in human cartilage homeostasis and chondrocyte survival by mediating the expression of major cartilage anabolic components, collagen 2(I) & aggrecan. The generation of the transcriptionally inactive 75-kDa fragment (75SirT1) via site-specific cleavage of FLSirT1 at amino acid 533 by cathepsin B is an anti-apoptotic mechanism to promote human osteoarthritic (OA) chondrocytes survival following exposure to proinflammatory cytokines. The 5SirT1 fragment is shown to associate with cytochrome c and likely blocks downstream apoptosome assembly. Phosphorylation plays an important role in modulating SirT1 activity and cellular functions. Multiple kinases and their corresponding SirT1 phosphorylation sites have been identified, including pT344 targeted by AMPK, pT530 and pS540 targeted by Cdk1/Cyclin B, pS46 (mouse) or pS47 (human) targeted by JNK1, pT522 targeted by DYRK1A & DYRK3, as well as pS27 & pS47 targeted by CaMKKβ.
~110 kDa observed

Immunogeno

Epitope: a.a. 1-131
GST-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α.

Applicazioni

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sirt1(Sir2) in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-131) is shown to be suitable also for Western blotting, immunoprecipitation, and ChIP applications.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-Sirt1 (Sir2) Antibody, Alexa Fluor™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of Sirt1 (Sir2).

Azioni biochim/fisiol

This antibody recognizes Sirt1.

Stato fisico

Affinity purified
Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Nota sulla preparazione

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sirt1(Sir2) in A431 cells.

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Non trovi il prodotto giusto?  

Prova il nostro Motore di ricerca dei prodotti.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Contenuto correlato

Post-Translational Modifications
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

"Mods – modifications – small alterations can have a big impact on form and function. In the motorsports world, stock vehicles are modified to enhance their performance. Modifications, or mods, to the engine, drive train, intake and exhaust components add up to provide phenomenal performance gains that can be measured as horsepower and torque increases, which yield a competitive advantage, and result in reduced run times. In biology, proteins undergo modifications that alter their function. Some proteins require the modifications in order to perform their function effectively, whether it’s a pro-protein that is cleaved to produce an active enzyme, or a protein that is phosphorylated to facilitate a signaling process. Other proteins, such as histones, undergo modifications that regulate gene expression and alter cellular function. There are several post translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination that impact protein function and activity. As a result, the analysis of proteins and their post-translational modifications are particularly important for the study of normal and disease-associated processes. New antibodies to detect phospho Histidines are now available from EMD Millipore."

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica