14-173-M

MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg

Name: MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg
Brand: MM
Price: 540 CHF

Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.

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14-173

CHF 540.00

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Informazioni su questo articolo

Codice UNSPSC:

12352202

eCl@ss:

32160405

NACRES:

NA.41

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Origine biologica

mouse

Livello qualitativo

100

Ricombinante

expressed in E. coli

PM

M_w 67.8 kDa

Produttore/marchio commerciale

Upstate^®

tecniche

activity assay: suitable (kinase)

N° accesso NCBI

NM_011949.3

N° accesso UniProt

P63085

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Questo articolo	14-550-M	14-439-M	14-198
Sigma-Aldrich 14-173-M MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg	Sigma-Aldrich 14-550-M MAP Kinase 2/Erk2 Protein, active, Human, 10 µg	Sigma-Aldrich 14-439-M MAP Kinase 1/Erk1 Protein, active, 10 µg	Sigma-Aldrich 14-198 MAP Kinase 2/Erk2 Protein, inactive, Mouse, 50 g
technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)	technique(s) activity assay: suitable (kinase)
biological source mouse	biological source human	biological source human	biological source mouse
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
UniProt accession no. P63085	UniProt accession no. P28482	UniProt accession no. P28482	UniProt accession no. P63085
mol wt M_w 67.8 kDa	mol wt M_w 67.8 kDa	mol wt M_w 69.9 kDa	mol wt M_w 67.8 kDa
manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®

Descrizione generale

N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).

Product Source: recombinant, mouse, full-length MAP Kinase 2

Azioni biochim/fisiol

Protein Target: MAPK2

Target Sub-Family: CMGC

Confezionamento

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.

Stato fisico

50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Glutathione agarose; Ni²⁺/NTA agarose

Nota sulla preparazione

Store in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.

Risultati analitici

Routinely evaluated by phosphorylation myelin basic protein

Altre note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pittogrammi

GHS07

Avvertenze

Warning

Indicazioni di pericolo

H317

Consigli di prudenza

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Identification of insulin-stimulated protein kinase-1 as the rabbit equivalent of rskmo-2. Identification of two threonines phosphorylated during activation by mitogen-activated protein kinase.

Sutherland, C, et al.

European Journal of Biochemistry, 212, 581-588 (1993)

Tyrosyl phosphorylation and activation of the myelin basic protein kinase p44mpk during sea star oocyte maturation.

Sanghera, J S, et al.

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Insulin- and mitogen-activated protein kinase-mediated phosphorylation and activation of peroxisome proliferator-activated receptor gamma.

B Zhang et al.

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Peroxisome proliferator-activated receptor (PPAR) gamma plays an important role in adipocyte differentiation and the regulation of adipocyte gene expression. Insulin also serves to promote adipogenesis. We report that insulin and a PPARgamma ligand (thiazolidinedione (TZD)) stimulate in a synergistic manner

alpha7 Nicotinic Acetylcholine Receptors Mediate {beta}-Amyloid Peptide-induced Tau Protein Phosphorylation

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Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.