17-10523
EZ-Magna NuCLEAR™ RIP (Native) Nuclear RNA-Binding Protein Immunoprecipitation Kit
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Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. In contrast, nuclear RIP with cross-linked chromatin can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. For less well understood proteins and protein complexes often both approaches are used.
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Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
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10 - Combustible liquids
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WGK 3
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