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10108090001

Roche

Peroxidase (POD)

Name: Peroxidase (POD)
Brand: ROCHE

grade I, from horseradish

Sinonimo/i:

POD, peroxidase

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Informazioni su questo articolo

UNSPSC Code:

12352204

Numero CE:

1.11.1.7 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg

Biological source:

horseradish

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Proprietà

biological source

horseradish

form

lyophilized (salt-free)

specific activity

≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg

packaging

pkg of 25,000 U

manufacturer/tradename

Roche

technique(s)

protein detection: suitable

optimum pH

6.0-6.5

shipped in

wet ice

storage temp.

2-8°C

Descrizione

General description

Donor:hydrogen-peroxide oxidoreductase

Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.

Application

Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.

Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H₂O₂ produced from disaggregated slug cells.

Biochem/physiol Actions

Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.

Preparation Note

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).

Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.

Analysis Note

Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase
Purity number: approximately 3.0 (A₄₀₃/A₂₇₅)

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Questo articolo	11428861001	SRE0082	P2088
Roche 10108090001 Peroxidase (POD)	Roche 11428861001 Peroxidase (POD), activated	Sigma-Aldrich SRE0082 Perossidasi	Sigma-Aldrich P2088 Perossidasi
description grade I, from horseradish	description from horseradish	description For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US, Type VI, powder, stabilized, ≥250 units/mg solid (using pyrogallol)	description Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)
specific activity ≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg	specific activity 800 units/mg protein (at 25 °C with ABTS and H₂O₂ as substrate (pH 5.0).)	specific activity ≥250 units/mg solid (using pyrogallol)	specific activity 200-300 units/mg solid (using pyrogallol)
biological source horseradish	biological source horseradish	biological source -	biological source horseradish
form lyophilized (salt-free)	form lyophilized	form powder, stabilized	form essentially salt-free, lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
optimum pH 6.0-6.5	optimum pH 6.0-6.5	optimum pH -	optimum pH -
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -

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Pittogrammi

GHS08

Parola di avvertenza

Danger

Codici di pericolo

H317,H334

Avvertenze di sicurezza

P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Classe di stoccaggio

11 - Combustible Solids

Che succede?

WGK 1

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)