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Merck

47608

Formaldeide

Molecular Biology, BioReagent, ≥36.0% in H2O (T)

Sinonimo/i:

Formalina

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Informazioni su questo articolo

Formula condensata:
HCHO
Numero CAS:
50-00-0
Peso molecolare:
30.03
Beilstein:
1209228
Numero MDL:
MFCD00003274
Codice UNSPSC:
12352114
ID PubChem:
329757328
NACRES:
NA.52

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Grado

Molecular Biology

Livello qualitativo

100

Densità del vapore

1.03 (vs air)

Tensione di vapore

52 mmHg ( 37 °C)

Nome Commerciale

BioReagent

Stato

liquid

Temp. autoaccensione

572 °F

contiene

~10% methanol as stabilizer

Concentrazione

≥36.0% in H2O (T)
36.0-38.0% (aldehyde method)

tecniche

electrophoresis: suitable

Impurezze

≤0.03% free acid (as HCOOH)

Residuo alla calcinazione

≤0.05% (as SO4)

pH

2.8-4.0

Densità

1.09 g/mL at 20 °C
1.09 g/mL at 25 °C (lit.)

Anioni in tracce

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤20 mg/kg

Cationi in tracce

Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Na: ≤100 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Zn: ≤1 mg/mL

λ

2 M in H2O

Assorbanza UV

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

Compatibilità

in accordance for electrophoresis of RNA in agarose gels

Temperatura di conservazione

room temp

Stringa SMILE

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2
WSFSSNUMVMOOMR-UHFFFAOYSA-N

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Questo articolo
F877547083-U1.04003
Formaldeide Molecular Biology, BioReagent, ≥36.0% in H2O (T)

Sigma-Aldrich

47608

Formaldeide

Formaldeide Molecular Biology, 36.5-38% in H2O

Sigma-Aldrich

F8775

Formaldeide

Formaldeide stabilized with methanol, ~37 wt. % in H2O, certified reference material

Supelco

47083-U

Formaldeide

Soluzione di formaldeide al 37% circa GR for analysis stabilized with about 10% methanol ACS,Reag. Ph Eur

Sigma-Aldrich

1.04003

Soluzione di formaldeide al 37% circa

technique(s)

electrophoresis: suitable

technique(s)

ChIP: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

grade

for molecular biology

grade

for molecular biology

grade

certified reference material

grade

ACS reagent

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

400

form

liquid

form

liquid

form

-

form

liquid

density

1.09 g/mL at 20 °C, 1.09 g/mL at 25 °C (lit.)

density

1.09 g/mL at 25 °C (lit.)

density

1.09 g/mL at 25 °C (lit.)

density

1.09 g/cm3 at 20 °C, 1.09 g/mL at 25 °C (lit.)

cation traces

Ca: ≤5 mg/kg, Co: ≤1 mg/kg, Cu: ≤1 mg/kg, K: ≤20 mg/kg, Mn: ≤1 mg/kg, Ni: ≤1 mg/kg, Zn: ≤1 mg/mL, Cd: ≤1 mg/kg, Fe: ≤1 mg/kg, Na: ≤100 mg/kg, Cr: ≤1 mg/kg, Pb: ≤1 mg/kg, Mg: ≤1 mg/kg

cation traces

-

cation traces

-

cation traces

-

Descrizione generale

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.[1]

Applicazioni

Formaldehyde solution is suitable for:
  • denaturation and separation of RNA molecules
  • anti-bacterial treatment
  • fixation of cells
Formaldehydesolution has been used:
  • in the fixation of human spermatogonial stem cell transplantation (SSCT).[2]
  • to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.[3]
  • in fixing zebra fish larvae for neutrophil staining.[4]

Azioni biochim/fisiol

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Esclusione di responsabilità

may contain precipitate (paraformaldehyde)

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Avvertenze

Danger

Classi di pericolo

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

Organi bersaglio

Eyes,Central nervous system, Respiratory system

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

133.0 °F - closed cup

Punto d’infiammabilità (°C)

56.11 °C - closed cup

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
BCCN1938
BCCM9304
BCCM0733
BCCL7726
BCCL1095

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Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation
Onofre J, et al.
Reproductive Biomedicine Online, 40(2), 261-269 (2020)
Formaldehyde
Gerberich, H R and Seaman, G C.
Kirk-Othmer Encyclopedia of Chemical Technology (2013)
Jin Qian et al.
Virology, 474, 10-18 (2014-12-03)
Interferon-induced transmembrane (IFITM) proteins inhibit the entry of a large number of viruses. Not surprisingly, many viruses are refractory to this inhibition. In this study, we report that different strains of HIV and SIV are inhibited by human IFITM proteins
John N Griffin et al.
PLoS genetics, 11(3), e1005018-e1005018 (2015-03-11)
The production of ribosomes is ubiquitous and fundamental to life. As such, it is surprising that defects in ribosome biogenesis underlie a growing number of symptomatically distinct inherited disorders, collectively called ribosomopathies. We previously determined that the nucleolar protein, NOL11
Y Jiang et al.
Oncogene, 34(50), 6079-6091 (2015-03-10)
Epstein-Barr virus (EBV) causes human lymphoid malignancies, and the EBV product latent membrane protein 1 (LMP1) has been identified as an oncogene in epithelial carcinomas such as nasopharyngeal carcinoma (NPC). EBV can epigenetically reprogram lymphocyte-specific processes and induce cell immortalization.
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