DAPI is suitable for staining bacterial chromosomes, including those of E. coli.
D9542
DAPI
for nucleic acid staining
DAPI
Sinonimo/i:
4′,6-diamidino-2-fenilindolo, 2-(4-amidinofenil)-6-indolocarbammidina, DAPI
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Informazioni su questo articolo
Formula empirica (notazione di Hill):
C16H15N5 · 2HCl
Numero CAS:
Peso molecolare:
350.25
Beilstein:
4894417
Numero CE:
Numero MDL:
Codice UNSPSC:
12352200
ID PubChem:
NACRES:
NA.52
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Servizio Tecnico
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Permettici di aiutartiGrado
Molecular Biology
Livello qualitativo
Saggio
≥98% (HPLC)
Stato
powder
tecniche
transfection: suitable
Solubilità
H2O: 20 mg/mL
PBS: insoluble
ε (coefficiente d’estinzione)
30 at 263 nm in H2O at 1 mM
Fluorescenza
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
Compatibilità
suitable for fluorescence
Temperatura di conservazione
2-8°C
Stringa SMILE
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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Categorie correlate
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Questo articolo | 10236276001 | 268298 | MBD0020 |
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability suitable for nucleic acid staining |
| grade for molecular biology | grade - | grade - | grade - |
| technique(s) transfection: suitable | technique(s) - | technique(s) - | technique(s) DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable |
| fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex ~359 nm, λem ~461 nm | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) |
| form powder | form powder | form solid | form liquid |
| solubility H2O: 20 mg/mL, PBS: insoluble | solubility - | solubility water: 5 mg/mL, ethanol: soluble, methanol: soluble | solubility - |
Descrizione generale
DAPI (4′,6-diamidino-2-fenilindolo diidrocloruro) è un colorante fluorescente permeabile alle cellule che si lega al DNA.
Potrebbe essere necessario ricorrere a calore o trattamento con ultrasuoni. La soluzione conservata al buio a temperatura ambiente o 4 °C dovrebbe essere stabile per 2-3 settimane.
Applicazioni
Adatto per
DAPI trova impiego:-
- Colorazione del DNA in gel di agarosio
- Analisi di cambiamenti nel DNA durante l′apoptosi
- Rilevamento di micoplasma
- fotoimpronte del DNA
- Colorazione delle cellule per immunofluorescenza
DAPI trova impiego:-
- nel monitoraggio rapido di contaminazione microbica[1]
- nella tecnica di rivelazione a bande dei cromosomi[2]
- nella rivelazione di cellule apoptotiche[3]
- Nella microscopia in fluorescenza per seguire il movimento del DisA (proteina per la scansione dell′integrità del DNA)) sul DNA di Bacillus subtilis [4]
- Per colorare granuli di polline maturo (0,5 mg/mL)[5]
DAPI è parecchie volte più sensibile del bromuro di etidio per colorare il DNA in gel di agarosio.
DAPI è parecchie volte più sensibile del bromuro di etidio per colorare il DNA in gel di agarosio. Può essere impiegato per fotoimpronte del DNA, rivelare sonde ibridizzate in applicazioni blotting visualizzando specificamente il complesso a doppio filamento, studiare i cambiamenti nel DNA e analizzare con la citometria di flusso il contenuto di DNA durante l′apoptosi. La colorazione via DAPI si è dimostrata un metodo di rivelazione sensibile e specifico dei micoplasma.
Azioni biochim/fisiol
Sonda fluorescente per DNA permeabile alle cellule che si attacca al solco minore. Si attacca al solco minore del DNA a doppio filamento (preferenzialmente a DNA ricco di T) a formare un complesso stabile con una fluorescenza circa 20 volte maggiore del DAPI da solo.
Esclusione di responsabilità
Tenere al riparo dalla luce.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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To apply fluorescent staining method for fast assessment of microbial quality of herbal medicines. The number of total bacteria and esterase-active bacteria on powdered traditional Chinese medicines were enumerated by fluorescent staining method using 6-carboxyfluorescein diacetate (6CFDA) and 4',6-diamidino-2-phenylindole (DAPI)
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Can this product be used to stain bacteria (E.coli) chromosome?
1 answer-
Helpful?
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What is the working dilution of DAPI that should be used to stain the nuclei of human cells?
1 answer-
Typical working concentration of DAPI used in most applications is 0.1μg/mL-10μg/mL. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/774/984/d9564pis.pdfPlease see the link below to review a sample protocol from Cold Spring Harbor:
https://cshprotocols.cshlp.org/content/2011/1/pdb.prot5556.longHelpful?
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what kind of the water used to dissolve DAPI? and what's the temperature should be dissolve the water?
1 answer-
This product is soluble at 20 mg/mL in water at ambient temperature. No specific water type or treatment is required. Deionized water is typically used for laboratory applications.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfHelpful?
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Can the stain concentration be used for staining fish scales and any animal cells for ICC?
1 answer-
Yes, DAPI can be used to stain fish scale nuclei. While specific data regarding the concentration and duration for staining fish scale nuclei is not available, it is suggested to start with a working concentration of around 0.5 mg/ml and optimize the staining for each specific cell type, as different cells may uptake the dye at varying rates. It is also advisable to try staining at both higher and lower concentrations. For staining mammalian cells, lower concentrations are typically used.
Helpful?
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What is the suggested concentration for product D9542-1MG?
1 answer-
The suggested concentration for product D9542-1MG, DAPI, is in the range of 0.5 to 5 ug/ml.
Helpful?
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What is the suitable DAPI for immunofluorescence staining of free-floating rat brain sections?
1 answer-
Several DAPI products are suitable for staining free-floating rat brain sections. The same DAPI used for staining mycoplasma, cultured cells, or other cell types can be used for this purpose. Any of the DAPI products listed on the website should be suitable for this use.
Helpful?
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肝細胞を染める染色プロトコルを教えてください。
1 answer-
Please see the link below to review the product datasheet, which includes basic protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfA protocol specifically for hepatocyte staining is not available. However, the publication shown at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671579/Helpful?
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Aside from the applications described on the product page, how is D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), used?
1 answer-
DAPI is used as a nuclear counterstain in our FISH protocol at 2 μg/mL in antifade mounting media. DAPI can also be used as a vital dye to stain mature pollen grains (0.5 μg/mL). DAPI can bind to RNA, but its selectivity for DNA over RNA is high. It appears that DAPI uses an intercalating binding mode with RNA that is AU selective (Biochem., 31, 3103 (1992)). Selectivity of DAPI for DNA over RNA is greater than that shown by ethidium bromide and propidium iodide (Nucleic Acid Research, 6, 3535 (1979))
Helpful?
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be dissolved?
1 answer-
This product is soluble in water at 20 mg/mL, but may require heat. DAPI does not dissolve readily in PBS, 0.1 M phosphate, or 0.1 M Tris (pH 7).
Helpful?
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be stored once in solution, and for how long would it be good?
1 answer-
Solutions stored in the dark at room temperature have been stable for 2-3 weeks, but solutions exposed to light for the same time period have shown considerable degradation. At 1 mg/mL, solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored at -20 °C.
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