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Merck

R0278

Tampone RIPA

RIPA Buffer

Sinonimo/i:

Radioimmunoprecipitation assay buffer

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R0278-50ML

CHF 103.00

R0278-500ML

CHF 638.00

CHF 103.00

Disponibile per la spedizione OGGIDettagli

Informazioni su questo articolo

Numero MDL:
MFCD02100484
Codice UNSPSC:
12161703
NACRES:
NA.56

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Descrizione

protease free

Livello qualitativo

200

Stato

liquid

Confezionamento

pkg of 50 mL
pkg of 500 mL

tecniche

immunoprecipitation (IP): suitable

Colore

colorless

pH

7.8-8.2 (25 °C, 1 ×)

Temperatura di conservazione

2-8°C

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Questo articolo
20-18811814389001T9285
Tampone RIPA

Sigma-Aldrich

R0278

Tampone RIPA

Tampone di lisi RIPA, 10X 100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.

Millipore

20-188

Tampone di lisi RIPA, 10X

Red Blood Cell Lysis Buffer solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions

Roche

11814389001

Red Blood Cell Lysis Buffer

Tampone Tris-EDTA 100X concentrate for Northern and Southern blotting

Sigma-Aldrich

T9285

Tampone Tris-EDTA

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

200

form

liquid

form

liquid

form

solution

form

liquid

pH

7.8-8.2 (25 °C, 1 ×)

pH

-

pH

-

pH

7.8-8.2 (1 × in water)

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

room temp

color

colorless

color

-

color

-

color

-

Descrizione generale

Il tampone RIPA (saggio di radioimmunoprecipitazione) viene fornito come soluzione pronta all′uso e non richiede alcuna preparazione. Esso minimizza il binding proteico aspecifico, mantenendo basso il rumore di fondo e rende possibili, nello stesso tempo, le interazioni più specifiche, consentendo lo studio di interazioni proteina-proteina di rilievo. È ampiamente usato in applicazioni come l′immunoprecipitazione poiché la maggior parte degli anticorpi e degli antigeni proteici non sono influenzati negativamente dai componenti di questo tampone.
Il tampone di saggio di radioimmunoprecipitazione (tampone RIPA) è un tampone di lisi usato per una rapida ed efficiente lisi cellulare e per la solubilizzazione delle proteine da cellule di mammifero in coltura aderenti e in sospensione.

Applicazioni

Il tampone RIPA consente un′efficiente lisi cellulare e solubilizzazione delle proteine evitando la degradazione delle stesse e l′interferenza con l′immunoreattività e l′attività biologica delle proteine. Inoltre, il tampone RIPA offre un ridotto rumore di fondo nell′immunoprecipitazione e nei saggi di pull-down molecolare. Compatibile con i gel per affinità Ezview™.
Il tampone RIPA è stato usato come reagente di lisi cellulare nei seguenti studi sperimentali:
  • fenotipizzazione profonda di tessuto periferico in base ai fibroblasti per facilitare la stratificazione meccanicista della malattia nel morbo di Parkinson sporadico (sPD).[1]
  • dimostrazione dell′inibizione della fosforilazione ossidativa (OXPHOS) che può attivare la necroptosi proteina MLKL (mixed lineage kinase domain-like)-dipendente in cellule epiteliali polmonari umane.[2]

Caratteristiche e vantaggi

1 mL di tampone RIPA è usato per la lisi delle cellule di una piastra di coltura da 100 mm (da 0,5 a 5 * 107 cellule) delle linee cellulari mammifere più aderenti.
Riduce al minimo le interazioni non specifiche da legame proteico per garantire un ridotto rumore di fondo.

Altre note

Soluzione pronta all′uso contenente NaCl 150 mM, IGEPAL® 1,0% CA-630, sodio desossicolato 0,5%, SDS 0,1%, Tris 50 mM, pH 8,0.

Note legali

EZview is a trademark of Sigma-Aldrich Co. LLC
IGEPAL is a registered trademark of Solvay

Prodotti correlati

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Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H319,H412

Classi di pericolo

Aquatic Chronic 3 - Eye Irrit. 2

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000504725
0000504726
0000504726
0000504725
0000437799

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CelLytic Protein Extraction Reagents
Product Information Sheet - R0278

Instructions

GC5, GC10, and Thunderbolt Competent Cells
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Questions

1–10 of 12 Questions  

  1. we bought a bottle of RIPA buffer and want to make alliquots of this bottle. Can we store the alliquots of the buffer by -20? Or is it better to keep them between 2°C-8°C ?

    1 answer

    1. It is recommended to store the product at 2–8C. Studies have not been performed on aliquots and to determine if refrigerated storage will extend the shelf life of this product. We would recommend searching the general research literature or contacting researchers who routinely use this product to see if solution stability information is available. If a recommended storage is not given in the literature, it is usually best to make solutions fresh for use. If you decide to store the aliquots at -20°C, we would recommend validating activity of the supplemented buffer after freezing and avoid any thawing/freezing cycle. Please refer to this technical bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  2. Is this RIPA lysis buffer capable of extracting nuclear protein in mammalian cells?

    1 answer

    1. Yes, RIPA buffer typically lyses cell nuclei. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  3. Can I use R0278 for human protein extract for lymphocytes?

    1 answer

    1. This product has not been tested for protein extract from human lymphocytes. References using RIPA buffer with human lymphocytes can be found here:
      https://www.sciencedirect.com/science/article/pii/S0021925818639675
      https://www.sciencedirect.com/science/article/pii/S000887491530037X?via%3Dihub

      Helpful?

  4. Can I use the Ripa Buffer to extract yeast protein and make the Bradford assay on it?

    1 answer

    1. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  5. How long can the RIPA buffer be stored in 4C? I stored it for almost 6 years, can I still use it?

    1 answer

    1. Regarding stability in the recommended temperature of 2-8 °C, this product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  6. Can this be used for Western blot experiments?

    1 answer

    1. Yes, this product would be suitable for western blotting.

      Helpful?

  7. Can Product R0278, RIPA buffer, be used on tissue samples, or only on cultured cells?

    1 answer

    1. R0278 can be used on tissue, if it is well homogenized.

      Helpful?

  8. How does Product R0278, RIPA buffer, compare to Product No. C2978 (CelLytic M)?

    1 answer

    1. RIPA buffer contains SDS, which is considered to be a harsh detergent; it can denature many proteins. C2978 is a buffered solution (pH 7.6) that contains a mild detergent; it is non-denaturing for most proteins.  The exact formulation of C2978, however, is proprietary.

      Helpful?

  9. I left the bottle of Product R0278, RIPA buffer, on my bench for about a week. Can I still use it?

    1 answer

    1. Yes, you should be able to use this product.  Although we recommend storage in the refrigerator at 2-8°C for long-term storage, Product No. R0278 may be kept at ambient temperature (our normal shipping condition) for a short time (approximately 2 weeks).

      Helpful?

  10. Does Product R0278, RIPA buffer, contain any protease inhibitors or phosphatase inhibitors?

    1 answer

    1. This buffer does not contain any protease or phosphatase inhibitors, however, they may be added to insure against possible proteolysis.

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  1. 1 Ratings-only review

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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