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S1797

SOC Medium

For use in transformation

Sinonimo/i:

Super Optimal broth

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Informazioni su questo articolo

NACRES:
NA.85
UNSPSC Code:
41106200
Prezzi e disponibilità al momento non sono disponibili
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grade

Molecular Biology

Quality Segment

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO4, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable, molecular cloning: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Features and Benefits

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

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Questo articolo
85469H8032M3409
SOC Medium For use in transformation

Sigma-Aldrich

S1797

SOC Medium

SOC Broth suitable for microbiology

Millipore

85469

SOC Broth

Hanahan’s Broth (SOB Medium) powder microbial growth medium

Sigma-Aldrich

H8032

Hanahan′s Broth (SOB Medium)

Magnesium sulfate solution Molecular Biology, 1.00 M±0.04 M

Sigma-Aldrich

M3409

Magnesium sulfate solution

description

For use in transformation

description

suitable for microbiology

description

powder microbial growth medium

description

Molecular Biology, 1.00 M±0.04 M

sterility

0.2 μm filtered

sterility

sterile (Filtered and Aseptic Handled)

sterility

non-sterile

sterility

0.2 μm filtered

application(s)

agriculture

application(s)

microbiology, food and beverages
life science and biopharma

application(s)

food and beverages
microbiology

application(s)

-

form

liquid

form

powder

form

powder

form

liquid

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for coliforms, nonselective for Escherichia coli

suitability

nonselective for coliforms, nonselective for Escherichia coli

suitability

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

Classe di stoccaggio

12 - Non Combustible Liquids

Che succede?

WGK 1

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

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Questions

1–4 of 4 Questions  

  1. That is the shelf life of SOC medium if kept refrigerated at 4C?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 2 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. Why is SOC Medium (S1797) used to recover bacterial cells before transformation?

    1 answer

    1. SOC is a rich bacterial broth that also contains glucose, which aids in the recovery process and prepares the cells for efficient transformation.  The use of other media, such as LB - Lennox may also be acceptable, but the recovery and transformation may not be as efficient.

      Helpful?

  4. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

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