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Merck

504955

Discovery® C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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Codice UNSPSC:
41115700
eCl@ss:
32110501

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Nome del prodotto

Colonna HPLC Discovery® C18, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

agenzia

suitable for USP L1

Livello qualitativo

100

Caratteristiche

endcapped

Produttore/marchio commerciale

Discovery®

Grado di funzionalizzazione

12% Carbon loading

Parametri

≤70 °C temp. range
400 bar pressure (5801 psi)

tecniche

HPLC: suitable

Lungh. × D.I.

15 cm × 4.6 mm

Area superficiale

300 m2/g

Copertura della superficie

3 μmol/m2

Impurezze

<10 ppm metals

Matrice

silica gel, high purity, spherical particle platform

Gruppo funzionale matrice

C18 (octadecyl) phase

Dimensione particelle

5 μm

Dimensione pori

180 Å

Intervallo di pH d’impiego

2-8

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Questo articolo
50494750497150494721
Colonna HPLC Discovery&#174; C18 5&#160;&#956;m particle size, L × I.D. 15&#160;cm × 4.6&#160;mm

Supelco

504955

Colonna HPLC Discovery® C18

Colonna HPLC Discovery&#174; C18 5&#160;&#956;m particle size, L × I.D. 5&#160;cm × 4.6&#160;mm

Supelco

504947

Colonna HPLC Discovery® C18

Colonna HPLC Discovery&#174; C18 5&#160;&#956;m particle size, L × I.D. 25&#160;cm × 4.6&#160;mm

Supelco

504971

Colonna HPLC Discovery® C18

Colonna HPLC Discovery&#174; C18 5&#160;&#956;m particle size, L × I.D. 5&#160;cm × 2.1&#160;mm

Supelco

50494721

Colonna HPLC Discovery® C18

L × I.D.

15 cm × 4.6 mm

L × I.D.

5 cm × 4.6 mm

L × I.D.

25 cm × 4.6 mm

L × I.D.

5 cm × 2.1 mm

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

pore size

180 Å

pore size

180 Å

pore size

180 Å

pore size

180 Å

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

Descrizione generale

Usare Discovery® C18 per qualsiasi metodo che specifichi una colonna C18. L′eccezionale profilo dei picchi, la riproducibilità e la stabilità ne fanno la colonna di elezione per tutti i metodi C18, da quelli di routine a quelli più critici.

Caratteristiche e vantaggi

  • Eccellente riproducibilità
  • Picchi eccezionalmente netti per analiti acidi e basici
  • Separazioni LC-MS stabili e a basso spurgo
  • Separazione di peptidi e piccole proteine
  • L′idrofobicità inferiore rispetto a molte colonne C18 confrontabili favorisce analisi più rapide.

Altre note

Scopri i reagenti LiChropur, ideali per analisi HPLC o LC-MS

Note legali

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm

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HPLC Analysis of Angiotensins on Discovery® C18, application for HPLC

G000595

HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery® C18 after SPE using Discovery® DSC-18, application for SPE, application for HPLC

G001056

HPLC Analysis of Barbiturates in Serum on Discovery® C18 after SPE using Discovery® DSC-18Lt, application for SPE, application for HPLC

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HPLC Analysis of 3-Methylpyrazole and 4-Methylpyrazole in Urine on Discovery® C18 after SPE using Discovery® DSC-SCX, application for SPE, application for HPLC

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G005684

HPLC Analysis of Caffeine Metabolites and Analogs on Discovery® C18, application for HPLC

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
298515-06
298515-05
298515-04
298515-03
298515-02

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T R Shantha Kumar et al.
Journal of pharmaceutical and biomedical analysis, 38(1), 173-179 (2005-05-24)
A simple, precise and accurate isocratic reverse-phase liquid chromatography method with programmed wavelength detection has been validated to quantify DRF-4367 and Phenol red, simultaneously for application in rat in situ single pass intestinal perfusion study to assess intestinal permeability of
Himal Paudel Chhetri et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 22(5), 483-487 (2014-12-05)
This study presents the optimization of a simple HPLC-UV method for the determination of metformin in human plasma. Ion pair separation followed by UV detection was performed on deproteinized human plasma samples. The separation was carried out on a Discovery
Abbas Khan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(24), 2423-2429 (2011-07-26)
A novel isocratic reversed-phase high performance liquid-chromatography/ultraviolet detection method for simultaneous determination of cefdinir and cefixime in human plasma was developed and validated after optimization of various chromatographic conditions and other experimental parameters. Sample preparation based on a simple extraction
Ramisetti Nageswara Rao et al.
Journal of separation science, 32(9), 1312-1322 (2009-04-29)
Modafinil, adrafinil and their related substances were synthesized and analyzed by RP-LC with ESI-MS/MS. The ionization mode, polarity, cone voltage, and chromatographic conditions were evaluated. The optimum LC-MS conditions to obtain fragment ions indispensable for identification of the structures were
Yoshinori Masukawa et al.
Journal of lipid research, 49(7), 1466-1476 (2008-03-25)
Ceramides (CERs) in human stratum corneum (SC) play physicochemical roles in determining barrier and water-holding functions of the skin, and specific species might be closely related to the regulation of keratinization, together with other CER-related lipids. Structures of those diverse
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Chromatograms

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. What is the  shipping solvent for Discovery® C18 HPLC Column, 504955?

    1 answer

    1. The shipping solvent for Discovery®C18 HPLC Column, 504955 is 66% Methanol: 34% Water.

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  3. How can I determine the shelf life / expiration / retest date of this product?

    0 answers

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