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Merck

504971

Discovery® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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Codice UNSPSC:
41115700
eCl@ss:
32110501

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Nome del prodotto

Colonna HPLC Discovery® C18, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

agenzia

suitable for USP L1

Livello qualitativo

100

Caratteristiche

endcapped

Produttore/marchio commerciale

Discovery®

Grado di funzionalizzazione

12% Carbon loading

Parametri

≤70 °C temp. range
400 bar pressure (5801 psi)

tecniche

HPLC: suitable

Lungh. × D.I.

25 cm × 4.6 mm

Area superficiale

300 m2/g

Copertura della superficie

3 μmol/m2

Impurezze

<10 ppm metals

Matrice

silica gel, high purity, spherical particle platform

Gruppo funzionale matrice

C18 (octadecyl) phase

Dimensione particelle

5 μm

Dimensione pori

180 Å

Intervallo di pH d’impiego

2-8

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Questo articolo
50494750495550494721
Colonna HPLC Discovery&#174; C18 5&#160;&#956;m particle size, L × I.D. 25&#160;cm × 4.6&#160;mm

Supelco

504971

Colonna HPLC Discovery® C18

Colonna HPLC Discovery&#174; C18 5&#160;&#956;m particle size, L × I.D. 5&#160;cm × 4.6&#160;mm

Supelco

504947

Colonna HPLC Discovery® C18

Colonna HPLC Discovery&#174; C18 5&#160;&#956;m particle size, L × I.D. 15&#160;cm × 4.6&#160;mm

Supelco

504955

Colonna HPLC Discovery® C18

Colonna HPLC Discovery&#174; C18 5&#160;&#956;m particle size, L × I.D. 5&#160;cm × 2.1&#160;mm

Supelco

50494721

Colonna HPLC Discovery® C18

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

L × I.D.

25 cm × 4.6 mm

L × I.D.

5 cm × 4.6 mm

L × I.D.

15 cm × 4.6 mm

L × I.D.

5 cm × 2.1 mm

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

Descrizione generale

Usare Discovery® C18 per qualsiasi metodo che specifichi una colonna C18. L′eccezionale profilo dei picchi, la riproducibilità e la stabilità ne fanno la colonna di elezione per tutti i metodi C18, da quelli di routine a quelli più critici.

Applicazioni

Discovery® C18 HPLC columns provide a classic reversed-phase selectivity and retention with excellent peak shape for all compounds featuring high stability and no-bleed properties for LC-MS applications. Typical applications are the separation of Aflatoxins, Aldehydes, Alkaloids, Antibiotics, Aromatic amines, Catecholamines, Esters, Lipids, Fatty acids, Flavonoids, Ketones, Nitrosamines, Nucleosides, Nucleotides, PCB, Peptides, Pesticides, Phenols, Phospholipids, Phthalates, Preservatives, Steroids, Sulfonamides, Sweeteners and Vitamins.

Caratteristiche e vantaggi

  • Eccellente riproducibilità
  • Picchi eccezionalmente netti per analiti acidi e basici
  • Separazioni LC-MS stabili e a basso spurgo
  • Separazione di peptidi e piccole proteine
  • L′idrofobicità inferiore rispetto a molte colonne C18 confrontabili favorisce analisi più rapide.

Altre note

Scoprite i reagenti LiChropur ideali per analisi HPLC o LC-MS

Note legali

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
299159-06
299159-05
299159-04
299159-03
299159-02

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T Nagano et al.
Journal of dental research, 88(9), 823-828 (2009-09-22)
Mmp-20 and Klk4 are the two key enamel proteases. Can both enzymes process amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis? We isolated Mmp-20 and Klk4 from developing pig teeth and used them
R Nageswara Rao et al.
Biomedical chromatography : BMC, 23(11), 1145-1150 (2009-05-16)
A highly sensitive and selective on-line two-dimensional reversed-phase liquid chromatography/electrospray ionization-tandem mass spectrometry (2D-LC-ESI/MS/MS) method was developed and validated to determine rifaximin in rat serum by direct injection. The 2D-LC-ESI/MS/MS system consisted of a restricted access media column for trapping
Yoshinori Masukawa et al.
Journal of lipid research, 49(7), 1466-1476 (2008-03-25)
Ceramides (CERs) in human stratum corneum (SC) play physicochemical roles in determining barrier and water-holding functions of the skin, and specific species might be closely related to the regulation of keratinization, together with other CER-related lipids. Structures of those diverse
Pei Yuan et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 15(42), 11319-11325 (2009-09-18)
This study systematically investigates periodic mesoporous organosilicas (PMOs) with controlled helical and concentric circular (CC) pore architectures prepared through a basic-catalyzed sol-gel process by using an achiral cationic surfactant trimethyloctadecylammonium bromide (C(18)TAB) as a structure-directing agent, perfluorooctanoic acid (PFOA) as
Pilar Heredia-Middleton et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 149(4), 552-556 (2008-02-01)
Heat shock protein 70 (HSP70, 70 kDa) is the most commonly expressed protein in response to thermal stress. The extent of its expression is associated with differences in environmental temperatures. We investigated the heat shock response in red blood cells
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Chromatograms

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. What is the shipping solvent of the column?

    1 answer

    1. The shipping solvent of the column is 66% methanol and 34% water.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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