341006

EDAC, Hydrochloride

EDAC HCl is a water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis.

• Synonyma: EDAC, Hydrochloride, EDCI, 1-Ethyl-3-(3ʹ-dimethylaminopropyl)carbodiimide, HCl
• Empirický vzorec (Hillův zápis): C₈H₁₇N₃ · xHCl
• Číslo CAS: 25952-53-8
• Molekulová hmotnost: 155.24 (free base basis)
• UNSPSC Code: 41116133
• NACRES: NA.21
• MDL number: MFCD00012503

Vlastnosti

• Quality Segment: 200
• description: RTECS - FF2200000
• assay: ≥98% (titration)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• color: white
• solubility: aqueous buffer: 2-5 mg/mL, water: 2-5 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: Cl.N(CCCN(CC)C#N)(C)C
• InChI: 1S/C8H17N3.ClH/c1-4-11(8-9)7-5-6-10(2)3;/h4-7H2,1-3H3;1H
• InChI key: FDXPUDRRFDHONO-UHFFFAOYSA-N

Popis

General description

Molar absorptivity (~212 nm; 50 mM phosphate buffer, pH 8):≥6800 M^-1cm^-1.

Water-soluble carbodiimide derivative. Used to modify NMDA receptors. Also useful for conjugating haptens to proteins or polypeptides.

Water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis. The major advantage of EDAC coupling is the easy removal of excess reagent and the corresponding urea by washing with dilute acid or water. Carbodiimides catalyze the formation of amide bonds, carboxylic acids, and amines by activating the carboxylate to form an O-acylurea. This intermediate can be attacked by an amine directly to form an amide. EDAC is released as a soluble urea derivative.

Packaging

Packaged under inert gas

Preparation Note

Avoid amine containing buffers.

Unstable in solution. Reconstitute just prior to use.

Other Notes

Chazot, P.L., et al. 1993. Biochem. Pharmacol. 45, 605.
Richardson, A., et al. 1992. Biochem. Pharmacol. 43, 1415.
Taniuchi, M., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1950.
Chase, J.W., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5480.
Williams, A., et al. 1981. J. Am. Chem. Soc.103, 7090.
Yamada, H., et al. 1981. Biochemistry20, 4836.
Thomas, J.O., et al. 1978. J. Mol. Biol.123, 149.
Ozawa, H. 1970. Biochemistry9, 2158.
Kopple, K.D., et al. 1962. J. Am. Chem. Soc.84, 4457.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Bezpečnostní informace

• Piktogramy: GHS06,GHS08,GHS09
• Výstražné slovo: Danger
• Cílové orgány: Stomach,large intestine,lymph node
• Skladovací třída: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• Kódy nebezpečí: H302,H311,H315,H317,H319,H373,H410
• Bezpečnostní pokyny: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral