T4038

Tris Acetate-EDTA buffer

DNase and RNase, none detected, BioReagent, suitable for electrophoresis, 10× concentrate

• Synonyma: TAE buffer
• NACRES: NA.25
• PubChem Substance ID: 329826782
• UNSPSC Code: 41105319
• MDL number: MFCD00236357

Vlastnosti

• Quality Segment: 200
• product line: BioReagent
• form: powder blend
• concentration: 8.5-10.5 ×
• impurities: DNase and RNase, none detected
• pH: 8.1-8.5
• solubility: water: 64.2 g/L, clear, colorless
• suitability: suitable for electrophoresis, suitable for gel electrophoresis (after dilution to working concentration)
• application(s): diagnostic assay manufacturing
• SMILES string: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• InChI key: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Popis

Application

Ready for use in gel electrophoresis after dilution to working concentrations.

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Packaged in pouches

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Produces a 10× concentrate (0.4 M Tris-acetate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

Bezpečnostní informace

• Piktogramy: GHS08
• Výstražné slovo: Warning
• Kódy nebezpečí: H373
• Bezpečnostní pokyny: P260 - P314 - P501
• Hazard Classifications: STOT RE 2 Inhalation
• Cílové orgány: Respiratory Tract
• Skladovací třída: 11 - Combustible Solids
• Co se děje?: WGK 3
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): dust mask type N95 (US), Eyeshields, Gloves