Merck
All Photos(1)

E8008

Sigma-Aldrich

Ethylendiamintetraessigsäure -Lösung

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):
EDTA -Lösung
Empirical Formula (Hill Notation):
C10H16N2O8
CAS Number:
Molecular Weight:
292.24
Beilstein:
1716295
MDL-Nummer:
eCl@ss:
39030907
PubChem Substanz-ID:
NACRES:
NA.75

Sterilität

sterile-filtered

Produktlinie

BioReagent

Form

solution

Konzentration

0.02% in DPBS (0.5 mM)

technique(s)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

pH-Wert

7.1-7.7

versandt in

wet ice

Lagertemp.

2-8°C

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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This Item
EDSEDE9884
product line

BioReagent

product line

BioUltra

product line

-

product line

-

form

solution

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

≤0.005% insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

pH

7.1-7.7

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

Allgemeine Beschreibung

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Anwendung

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

Physikalische Form

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt F

Not applicable

Flammpunkt C

Not applicable


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