This kit provides sufficient reagents to perform 18 chloroplast isolation procedures, using 30 grams of starting plant tissue, as described in the protocol. Please see the link below to review the product datasheet:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/229/cpisopis-ms.pdf
CPISO
Chloroplast Isolation Kit
isolation of intact chloroplasts from leaves
Synonyme(s) :
Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation
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| Taille/Référence | Disponibilité | Prix |
|---|---|---|
1 kit | Disponible dès aujourd'huiDeSt QuentinFallavier | 971,00 € |
A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352200
971,00 €
Disponible dès aujourd'huiDétails
Service technique
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technique(s)
centrifugation: suitable, fractionation: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.
Application
Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.
The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.
Features and Benefits
- Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
- Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
- Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
- Convenient format - Saves time and reduces waste
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Cet article | |||
|---|---|---|---|
| description isolation of intact chloroplasts from leaves | description isolate peroxisomes from tissues and cells | description sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells | description isolation of intact ER from mammalian soft tissues and cultured cells |
| technique(s) centrifugation: suitable | technique(s) fractionation: suitable, centrifugation: suitable | technique(s) fractionation: suitable, centrifugation: suitable | technique(s) fractionation: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
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10 - Combustible liquids
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