05-362

Anti-Cyclin D1/2 Antibody, clone 5D4

clone 5D4, Upstate^®, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5D4, monoclonal
• species reactivity: rat, mouse, human, hamster
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_001759; NM_053056
• UniProt accession no.: P24385
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CCND1(595)

Description

General description

34/36 kDa

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. Cyclin D2 is also a G1 cyclin required for G1 phase progression and is a strong candidate for a proto oncogene. Cyclin D2 can phosphorylate pRB when associated with cdk 4 and / or cdk 6.

Immunogen

Recombinant human cyclin D1

Application

Anti-Cyclin D1/2 Antibody, clone 5D4 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin D1/2 & has been validated in ICC, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

Recognizes p36 cyclin D1 and p34 cyclin D2; no cross-reactivity with cyclin A, B or D3.

Physical form

Format: Purified

Protein A purified

Protein A purified IgG2a in 50% storage buffer containing PBS, pH 7.2 and 50% glycerol.

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on RIPA lysate from human A431 cells

Other Notes

Replaces: 04-1151

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable