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Merck

UFC30GV0S

Ultrafree® Centrifugal Filter, 0.5 mL Sample Volume

pore size 0.22 μm, PVDF membrane (hydrophilic)

Synonym(s):

Centrifuge filter

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50 EA

HUF 90,200.00

HUF 90,200.00


Estimated to ship TODAYDetails



About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

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material

PVDF membrane (hydrophilic)
polypropylene

Quality Level

sterility

sterile

feature

holdup volume 5 μL

packaging

pkg of 50 ea

manufacturer/tradename

Ultrafree®

parameter

0.5 mL sample volume
50 °C max. temp.

technique(s)

protein extraction: suitable
protein purification: suitable

L

45 mm

diam.

10.6 mm

filtration area

0.2 cm2

working volume

0.5 mL

matrix

Durapore®

pore size

0.22 μm pore size

application(s)

sample preparation

shipped in

ambient

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This Item
UFC40GV0SUFC30VVUFC30DV25
parameter

0.5 mL sample volume, 50 °C max. temp.

parameter

2 mL sample volume, 50 °C max. temp.

parameter

0.5 mL sample volume, 50 °C max. temp.

parameter

0.5 mL sample volume, 50 °C max. temp.

pore size

0.22 μm pore size

pore size

0.22 μm pore size

pore size

0.1 μm pore size

pore size

0.65 μm pore size

material

PVDF membrane (hydrophilic), polypropylene

material

PVDF membrane (hydrophilic), polypropylene

material

PVDF membrane (hydrophilic), polypropylene

material

PVDF membrane (hydrophilic), polypropylene

manufacturer/tradename

Ultrafree®

manufacturer/tradename

Ultrafree®

manufacturer/tradename

Ultrafree®

manufacturer/tradename

Ultrafree®

sterility

sterile

sterility

sterile

sterility

non-sterile

sterility

non-sterile

feature

holdup volume 5 μL

feature

holdup volume 10 μL

feature

holdup volume 5 μL

feature

holdup volume 5 μL

General description

Ultrafree®-MC (0.5 mL) and Ultrafree®-CL (2 mL) centrifugal devices are single-use, disposable filters used for removing particulates from aqueous biological solutions. These devices are available in two processing volumes with a range of microporous membranes (from 0.1 to 5.0 m) for fast filtration and highly reproducible performance. Pre-sterilized units are also available. Use in fixed-angle rotors for 1.5 mL tubes (-MC) and 15 mL tubes (CL).

Filter Code: GVPP
Filter Type: Microfiltration

Application

Ultrafree®-MC GV Centrifugal Filter has been used


  • to filter cell lysates after centrifugation for Label-free mass spectrometry
  • for filtering extracted enzymes in the buffer for preparing imaging solution for cellular direct stochastic optical reconstruction microscopy (dSTORM) and DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) imaging
  • to purify viral solution during transfection studies
  • to filter heart tissue homogenates for preparation of the sample for high-pressure liquid chromatography (HPLC) analysis

Features and Benefits

  • Used for removal of particles and precipitates from aqueous and some solvent-based samples
  • Ideal for use with protein and nucleic acid solutions
  • Used for removal of particles and precipitates from aqueous and some solvent-based samples
  • Ideal for use with protein and nucleic acid solutions

Physical form

Color Code: Yellow

Legal Information

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Ultrafree is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Beta2-glycoprotein I can exist in 2 conformations: implications for our understanding of the antiphospholipid syndrome.
Agar, Cetin, et al.
Blood, 116, 1336-1343 (2010)
Robert Player et al.
BMC genomics, 21(1), 166-166 (2020-02-19)
The state-of-the-art in nucleic acid based biodetection continues to be polymerase chain reaction (PCR), and many real-time PCR assays targeting biodefense pathogens for biosurveillance are in widespread use. These assays are predominantly singleplex; i.e. one assay tests for the presence
Cetin Agar et al.
Blood, 117(25), 6939-6947 (2011-04-02)
Sepsis is a systemic host response to invasive infection by bacteria. Despite treatment with antibiotics, current mortality rates are in the range of 20%-25%, which makes sepsis the most important cause of death in intensive care. Gram-negative bacteria are a

Protocols

Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step

Questions

  1. Can these be vortexed? For example if I want to resuspend the concentrated molecules in another buffer.

    1 answer
    1. Yes, Ultrafree tubes can be vortexed.

      Helpful?

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