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Merck

A9731

Albumin human

Cellastim S, recombinant, expressed in rice, lyophilized powder, suitable for cell culture, low endotoxin, ≥96% (SDS-PAGE)

Albumin human

Synonym(s):

Cellastim S, rHSA, rHSA

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HUF 55,000.00

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HUF 186,000.00

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HUF 370,000.00

HUF 55,000.00


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About This Item

Empirical Formula (Hill Notation):
C2936H4624N786O889S41
CAS Number:
Molecular Weight:
66471.53
MDL number:
UNSPSC Code:
12352202
eCl@ss:
42010201
NACRES:
NA.25

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biological source

human

recombinant

expressed in rice

Assay

≥96% (SDS-PAGE)

form

lyophilized powder

mol wt

monomer ~67 kDa

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/mg protein

UniProt accession no.

storage temp.

2-8°C

SMILES string

FC(F)(F)C(F)(F)C(F)(F)F

InChI

1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11

InChI key

QYSGYZVSCZSLHT-UHFFFAOYSA-N

Gene Information

human ... ALB(213)

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1 of 4

This Item
A9511A5843Y0001913
biological source

human

biological source

human

biological source

human

biological source

-

assay

≥96% (SDS-PAGE)

assay

≥97% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

assay

-

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, tissue culture: suitable, western blot: suitable

technique(s)

activity assay: suitable

technique(s)

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

This recombinant variant is recommended for use in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells.

Biochem/physiol Actions

Albumins are soluble monomeric proteins found in the body fluids and tissues of animals and in some plant seeds. Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones. Serum albumins are also vital in regulating the colloidal osmotic pressures of blood.

Features and Benefits

Cellastim is a well defined, animal-free recombinant human albumin (rHA). Designed for cell culture applications, Cellastim is a powerful media supplement that outperforms plasma-derived human albumin, bovine serum albumin, and other sources of recombinant human albumin. Cellastim delivers consistent performance, not the variability associated with hydrolysates and extracts. Cellastim is ideally suited for use in cell culture media for Chinese Hamster Ovary (CHO), hybridoma, VERO, stem cells and primary cells.

Advantages
  • Eliminates animal-derived additives
  • Safe and regulatory friendly
  • Proven effective in enhancing stem cell growth in embryonic, mesenchymal, neural, cardiac, liver, kidney, keratinocyte and fibroblast stem cell lines
  • Maintains stem cells in an undifferentiated state
  • Eliminates coating of culture plates for adherent cell lines
  • Provides well-defined and consistent stem cell medium

Analysis Note

Assayed by SDS-PAGE

Other Notes

1). Patent Rights


EP2230311


U.S. Patent No. 10618951


U.S. Patent No. 10981974


U.S. Patent No. 11492389


2). Registered Trademark Rights


U.S. Reg. 5696531


International Reg. 1456207
To view more information on Cellastim download the Cellastim Brochure, Cellastim CHO Poster, and Cellastim hybridoma Poster.
View more information on human serum albumin.

Legal Information

rHSA manufactured in the USA by InVitria US Pat 10618951, 10981974, and 11492389.
Cellastim is a trademark of InVitria

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Junfeng Zhu et al.
Journal of pharmaceutical and biomedical analysis, 134, 100-107 (2016-11-28)
Danhong injection (DHI) is a widely used Chinese medicine injection (CMI) for the clinical treatment of cardiovascular and cerebrovascular diseases. In this study, a simple and efficient in vitro method based on ultrafiltration LC-MS and molecular modeling has been developed
Yongjia Fan et al.
ACS biomaterials science & engineering, 3(8), 1510-1518 (2016-08-14)
The development of platforms for the expansion and directed differentiation of human pluripotent stem cells (hPSCs) in large quantities under xeno-free conditions is a key step toward the realization of envisioned stem cell-based therapies. Microcarrier bioreactors afford great surface-to-volume ratio
D Moreno et al.
Theriogenology, 84(7), 1053-1060 (2015-08-19)
The aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-β1, and PDGF-BB), and other molecules with
Yasuhiro Kawai et al.
PloS one, 13(5), e0196655-e0196655 (2018-05-26)
Serum albumin is the major intravascular antioxidant. Though oxidative stress plays an important role in the pathophysiology of Immunoglobulin A nephropathy (IgAN), the association between serum albumin and the progression of IgAN is not entirely understood. This retrospective cohort study
Wireeya Chawjiraphan et al.
MethodsX, 7, 101114-101114 (2020-11-17)
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of

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Questions

1–2 of 2 Questions  
  1. "Does this HSA product contain free thiol at Cys34 (i.e., mercaptalbumin form)?"

    1 answer
    1. Unfortunately, we do not separately verify the free thiol % for this product. Therefore, we are unable to provide any information. We apologize for not being able to assist you.

      Helpful?

  2. Hello, does this construct include any affinity tags? Does it contain glycosylation?

    1 answer
    1. A9731 is free from affinity tags and it is glysylated. We have no information on glycosylations sites.

      Helpful?

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